Experiment: Def Vancomycin 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0147 and Psest_0148 are separated by 88 nucleotides Psest_0148 and Psest_0149 are separated by 11 nucleotides Psest_0149 and Psest_0150 are separated by 26 nucleotides
Psest_0147: Psest_0147 - Acetyltransferase, GNAT family., at 146,793 to 147,191
_0147
Psest_0148: Psest_0148 - nitrate transport ATP-binding subunits C and D, at 147,280 to 148,062
_0148
Psest_0149: Psest_0149 - nitrate ABC transporter, permease protein, at 148,074 to 148,943
_0149
Psest_0150: Psest_0150 - ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components, at 148,970 to 150,301
_0150
Position (kb)
148
149 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 147.087 kb on - strand, within Psest_0147 at 147.091 kb on + strand, within Psest_0147 at 147.091 kb on + strand, within Psest_0147 at 147.092 kb on - strand, within Psest_0147 at 147.124 kb on + strand, within Psest_0147 at 147.124 kb on + strand, within Psest_0147 at 147.124 kb on + strand, within Psest_0147 at 147.125 kb on - strand, within Psest_0147 at 147.125 kb on - strand, within Psest_0147 at 147.125 kb on - strand, within Psest_0147 at 147.125 kb on - strand, within Psest_0147 at 147.125 kb on - strand, within Psest_0147 at 147.125 kb on - strand, within Psest_0147 at 147.145 kb on - strand, within Psest_0147 at 147.181 kb on + strand at 147.181 kb on + strand at 147.181 kb on + strand at 147.182 kb on - strand at 147.231 kb on + strand at 147.338 kb on + strand at 147.338 kb on + strand at 147.339 kb on - strand at 147.339 kb on - strand at 147.339 kb on - strand at 147.662 kb on + strand, within Psest_0148 at 147.662 kb on + strand, within Psest_0148 at 147.663 kb on - strand, within Psest_0148 at 147.783 kb on - strand, within Psest_0148 at 148.055 kb on + strand at 148.055 kb on + strand at 148.055 kb on + strand at 148.055 kb on + strand at 148.055 kb on + strand at 148.055 kb on + strand at 148.055 kb on + strand at 148.055 kb on + strand at 148.056 kb on - strand at 148.056 kb on - strand at 148.056 kb on - strand at 148.056 kb on - strand at 148.056 kb on - strand at 148.056 kb on - strand at 148.056 kb on - strand at 148.056 kb on - strand at 148.056 kb on - strand at 148.156 kb on + strand at 148.156 kb on + strand at 148.156 kb on + strand at 148.156 kb on + strand at 148.157 kb on - strand at 148.291 kb on - strand, within Psest_0149 at 148.306 kb on + strand, within Psest_0149 at 148.307 kb on - strand, within Psest_0149 at 148.307 kb on - strand, within Psest_0149 at 148.307 kb on - strand, within Psest_0149 at 148.307 kb on - strand, within Psest_0149 at 148.307 kb on - strand, within Psest_0149 at 148.307 kb on - strand, within Psest_0149 at 148.307 kb on - strand, within Psest_0149 at 148.307 kb on - strand, within Psest_0149 at 148.307 kb on - strand, within Psest_0149 at 148.342 kb on + strand, within Psest_0149 at 148.627 kb on + strand, within Psest_0149 at 148.699 kb on + strand, within Psest_0149 at 148.699 kb on + strand, within Psest_0149 at 148.699 kb on + strand, within Psest_0149 at 148.700 kb on - strand, within Psest_0149 at 148.700 kb on - strand, within Psest_0149 at 149.004 kb on + strand at 149.005 kb on - strand at 149.235 kb on + strand, within Psest_0150 at 149.235 kb on + strand, within Psest_0150 at 149.256 kb on + strand, within Psest_0150 at 149.340 kb on + strand, within Psest_0150 at 149.340 kb on + strand, within Psest_0150 at 149.341 kb on - strand, within Psest_0150 at 149.475 kb on + strand, within Psest_0150 at 149.475 kb on + strand, within Psest_0150 at 149.475 kb on + strand, within Psest_0150 at 149.475 kb on + strand, within Psest_0150 at 149.476 kb on - strand, within Psest_0150 at 149.544 kb on + strand, within Psest_0150 at 149.829 kb on + strand, within Psest_0150 at 149.935 kb on - strand, within Psest_0150
Per-strain Table
Position Strand Gene LocusTag Fraction Def Vancomycin 0.016 mg/ml remove 147,087 - Psest_0147 0.74 -1.8 147,091 + Psest_0147 0.75 -0.2 147,091 + Psest_0147 0.75 +0.6 147,092 - Psest_0147 0.75 +0.3 147,124 + Psest_0147 0.83 -1.7 147,124 + Psest_0147 0.83 +2.1 147,124 + Psest_0147 0.83 +0.5 147,125 - Psest_0147 0.83 -0.0 147,125 - Psest_0147 0.83 -3.0 147,125 - Psest_0147 0.83 +0.8 147,125 - Psest_0147 0.83 -1.7 147,125 - Psest_0147 0.83 -1.2 147,125 - Psest_0147 0.83 -0.1 147,145 - Psest_0147 0.88 -0.8 147,181 + +0.6 147,181 + +1.5 147,181 + +2.1 147,182 - +0.3 147,231 + +0.2 147,338 + -1.0 147,338 + +0.0 147,339 - -0.4 147,339 - +0.7 147,339 - +0.7 147,662 + Psest_0148 0.49 -0.1 147,662 + Psest_0148 0.49 -0.9 147,663 - Psest_0148 0.49 +0.1 147,783 - Psest_0148 0.64 -1.9 148,055 + +0.1 148,055 + +0.2 148,055 + +0.4 148,055 + +0.5 148,055 + -0.2 148,055 + +0.0 148,055 + -1.2 148,055 + +0.5 148,056 - +0.3 148,056 - +0.4 148,056 - +1.2 148,056 - -0.6 148,056 - -0.1 148,056 - +0.9 148,056 - +0.2 148,056 - -0.5 148,056 - +0.3 148,156 + -0.2 148,156 + +0.4 148,156 + +1.3 148,156 + +0.9 148,157 - -0.9 148,291 - Psest_0149 0.25 +0.3 148,306 + Psest_0149 0.27 -1.4 148,307 - Psest_0149 0.27 +0.7 148,307 - Psest_0149 0.27 +0.8 148,307 - Psest_0149 0.27 +0.0 148,307 - Psest_0149 0.27 +1.0 148,307 - Psest_0149 0.27 +1.3 148,307 - Psest_0149 0.27 +0.9 148,307 - Psest_0149 0.27 +1.3 148,307 - Psest_0149 0.27 -0.1 148,307 - Psest_0149 0.27 +0.3 148,342 + Psest_0149 0.31 +1.6 148,627 + Psest_0149 0.64 +0.6 148,699 + Psest_0149 0.72 +1.2 148,699 + Psest_0149 0.72 -0.2 148,699 + Psest_0149 0.72 +0.7 148,700 - Psest_0149 0.72 -0.4 148,700 - Psest_0149 0.72 -0.0 149,004 + +2.1 149,005 - +0.2 149,235 + Psest_0150 0.20 +1.2 149,235 + Psest_0150 0.20 -1.4 149,256 + Psest_0150 0.21 +0.0 149,340 + Psest_0150 0.28 -0.3 149,340 + Psest_0150 0.28 -0.8 149,341 - Psest_0150 0.28 +1.8 149,475 + Psest_0150 0.38 +1.0 149,475 + Psest_0150 0.38 +0.6 149,475 + Psest_0150 0.38 -1.6 149,475 + Psest_0150 0.38 +1.3 149,476 - Psest_0150 0.38 +0.1 149,544 + Psest_0150 0.43 +0.3 149,829 + Psest_0150 0.64 +0.6 149,935 - Psest_0150 0.72 +0.9
Or see this region's nucleotide sequence