Experiment: Def Phosphomycin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0404 and Psest_0405 overlap by 4 nucleotides Psest_0405 and Psest_0406 overlap by 41 nucleotides Psest_0406 and Psest_0407 are separated by 83 nucleotides
Psest_0404: Psest_0404 - phytoene desaturase, at 439,233 to 440,738
_0404
Psest_0405: Psest_0405 - Phytoene/squalene synthetase, at 440,735 to 441,661
_0405
Psest_0406: Psest_0406 - Fatty acid hydroxylase superfamily., at 441,621 to 442,154
_0406
Psest_0407: Psest_0407 - Dioxygenases related to 2-nitropropane dioxygenase, at 442,238 to 443,281
_0407
Position (kb)
441
442
443 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 440.622 kb on + strand at 440.622 kb on + strand at 440.622 kb on + strand at 440.622 kb on + strand at 440.622 kb on + strand at 440.622 kb on + strand at 440.622 kb on + strand at 440.622 kb on + strand at 440.622 kb on + strand at 440.623 kb on - strand at 440.623 kb on - strand at 440.623 kb on - strand at 440.623 kb on - strand at 440.623 kb on - strand at 440.623 kb on - strand at 440.623 kb on - strand at 440.623 kb on - strand at 440.623 kb on - strand at 440.623 kb on - strand at 440.623 kb on - strand at 440.623 kb on - strand at 440.624 kb on + strand at 440.849 kb on + strand, within Psest_0405 at 440.849 kb on + strand, within Psest_0405 at 440.975 kb on + strand, within Psest_0405 at 440.975 kb on + strand, within Psest_0405 at 440.976 kb on - strand, within Psest_0405 at 441.099 kb on - strand, within Psest_0405 at 441.099 kb on - strand, within Psest_0405 at 441.099 kb on - strand, within Psest_0405 at 441.131 kb on + strand, within Psest_0405 at 441.131 kb on + strand, within Psest_0405 at 441.291 kb on - strand, within Psest_0405 at 441.349 kb on - strand, within Psest_0405 at 441.407 kb on + strand, within Psest_0405 at 441.411 kb on - strand, within Psest_0405 at 441.791 kb on + strand, within Psest_0406 at 441.838 kb on + strand, within Psest_0406 at 441.839 kb on - strand, within Psest_0406 at 441.839 kb on - strand, within Psest_0406 at 441.886 kb on + strand, within Psest_0406 at 441.886 kb on + strand, within Psest_0406 at 441.898 kb on + strand, within Psest_0406 at 441.994 kb on + strand, within Psest_0406 at 441.994 kb on + strand, within Psest_0406 at 442.075 kb on + strand, within Psest_0406 at 442.075 kb on + strand, within Psest_0406 at 442.075 kb on + strand, within Psest_0406 at 442.075 kb on + strand, within Psest_0406 at 442.075 kb on + strand, within Psest_0406 at 442.075 kb on + strand, within Psest_0406 at 442.075 kb on + strand, within Psest_0406 at 442.075 kb on + strand, within Psest_0406 at 442.075 kb on + strand, within Psest_0406 at 442.075 kb on + strand, within Psest_0406 at 442.075 kb on + strand, within Psest_0406 at 442.075 kb on + strand, within Psest_0406 at 442.075 kb on + strand, within Psest_0406 at 442.076 kb on - strand, within Psest_0406 at 442.076 kb on - strand, within Psest_0406 at 442.076 kb on - strand, within Psest_0406 at 442.076 kb on - strand, within Psest_0406 at 442.076 kb on - strand, within Psest_0406 at 442.076 kb on - strand, within Psest_0406 at 442.076 kb on - strand, within Psest_0406 at 442.076 kb on - strand, within Psest_0406 at 442.076 kb on - strand, within Psest_0406 at 442.076 kb on - strand, within Psest_0406 at 442.076 kb on - strand, within Psest_0406 at 442.076 kb on - strand, within Psest_0406 at 442.076 kb on - strand, within Psest_0406 at 442.137 kb on - strand at 442.154 kb on + strand at 442.154 kb on + strand at 442.155 kb on - strand at 442.155 kb on - strand at 442.231 kb on + strand at 442.495 kb on + strand, within Psest_0407 at 442.566 kb on + strand, within Psest_0407 at 442.577 kb on + strand, within Psest_0407 at 442.578 kb on - strand, within Psest_0407 at 442.578 kb on - strand, within Psest_0407 at 442.578 kb on - strand, within Psest_0407 at 442.578 kb on - strand, within Psest_0407 at 442.583 kb on + strand, within Psest_0407 at 442.584 kb on - strand, within Psest_0407 at 443.091 kb on + strand, within Psest_0407 at 443.091 kb on + strand, within Psest_0407 at 443.091 kb on + strand, within Psest_0407 at 443.092 kb on - strand, within Psest_0407
Per-strain Table
Position Strand Gene LocusTag Fraction Def Phosphomycin 0.125 mg/ml remove 440,622 + -0.0 440,622 + -0.9 440,622 + +0.4 440,622 + +0.4 440,622 + -0.4 440,622 + +0.6 440,622 + -1.0 440,622 + -0.6 440,622 + -0.4 440,623 - +1.0 440,623 - +2.5 440,623 - +1.0 440,623 - +0.4 440,623 - +0.4 440,623 - -0.8 440,623 - -1.0 440,623 - +1.0 440,623 - -0.0 440,623 - -0.8 440,623 - +1.0 440,623 - -0.3 440,624 + -0.2 440,849 + Psest_0405 0.12 -1.6 440,849 + Psest_0405 0.12 -0.5 440,975 + Psest_0405 0.26 -0.0 440,975 + Psest_0405 0.26 -0.6 440,976 - Psest_0405 0.26 -1.5 441,099 - Psest_0405 0.39 -1.4 441,099 - Psest_0405 0.39 +1.0 441,099 - Psest_0405 0.39 -1.0 441,131 + Psest_0405 0.43 +1.0 441,131 + Psest_0405 0.43 +0.4 441,291 - Psest_0405 0.60 -1.1 441,349 - Psest_0405 0.66 -0.3 441,407 + Psest_0405 0.72 -0.5 441,411 - Psest_0405 0.73 -0.1 441,791 + Psest_0406 0.32 +0.4 441,838 + Psest_0406 0.41 +1.0 441,839 - Psest_0406 0.41 -2.1 441,839 - Psest_0406 0.41 -0.5 441,886 + Psest_0406 0.50 -0.0 441,886 + Psest_0406 0.50 -0.8 441,898 + Psest_0406 0.52 +1.0 441,994 + Psest_0406 0.70 -0.4 441,994 + Psest_0406 0.70 -0.5 442,075 + Psest_0406 0.85 +1.0 442,075 + Psest_0406 0.85 -0.8 442,075 + Psest_0406 0.85 +0.2 442,075 + Psest_0406 0.85 -0.0 442,075 + Psest_0406 0.85 +1.4 442,075 + Psest_0406 0.85 +1.0 442,075 + Psest_0406 0.85 -0.2 442,075 + Psest_0406 0.85 -0.8 442,075 + Psest_0406 0.85 +0.4 442,075 + Psest_0406 0.85 -2.0 442,075 + Psest_0406 0.85 +2.5 442,075 + Psest_0406 0.85 +0.4 442,075 + Psest_0406 0.85 -0.7 442,076 - Psest_0406 0.85 +1.5 442,076 - Psest_0406 0.85 +0.4 442,076 - Psest_0406 0.85 +0.7 442,076 - Psest_0406 0.85 +1.0 442,076 - Psest_0406 0.85 -1.6 442,076 - Psest_0406 0.85 +0.4 442,076 - Psest_0406 0.85 +1.6 442,076 - Psest_0406 0.85 +2.0 442,076 - Psest_0406 0.85 +0.4 442,076 - Psest_0406 0.85 -0.4 442,076 - Psest_0406 0.85 -1.3 442,076 - Psest_0406 0.85 +0.5 442,076 - Psest_0406 0.85 -0.4 442,137 - +1.0 442,154 + +0.4 442,154 + -0.4 442,155 - +0.0 442,155 - +2.0 442,231 + +0.6 442,495 + Psest_0407 0.25 +1.3 442,566 + Psest_0407 0.31 -0.3 442,577 + Psest_0407 0.32 -0.0 442,578 - Psest_0407 0.33 -0.9 442,578 - Psest_0407 0.33 -2.2 442,578 - Psest_0407 0.33 +1.1 442,578 - Psest_0407 0.33 +0.4 442,583 + Psest_0407 0.33 +0.6 442,584 - Psest_0407 0.33 -0.4 443,091 + Psest_0407 0.82 +0.2 443,091 + Psest_0407 0.82 -0.4 443,091 + Psest_0407 0.82 +1.2 443,092 - Psest_0407 0.82 +0.8
Or see this region's nucleotide sequence