Experiment: Def Phosphomycin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2316 and Psest_2317 are separated by 154 nucleotides Psest_2317 and Psest_2318 are separated by 93 nucleotides
Psest_2316: Psest_2316 - phosphoenolpyruvate synthase, at 2,466,611 to 2,468,980
_2316
Psest_2317: Psest_2317 - Uncharacterized protein conserved in bacteria, at 2,469,135 to 2,469,953
_2317
Psest_2318: Psest_2318 - probable AcnD-accessory protein PrpF, at 2,470,047 to 2,471,234
_2318
Position (kb)
2469
2470 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2468.190 kb on + strand, within Psest_2316 at 2468.190 kb on + strand, within Psest_2316 at 2468.191 kb on - strand, within Psest_2316 at 2468.271 kb on + strand, within Psest_2316 at 2468.272 kb on - strand, within Psest_2316 at 2468.966 kb on + strand at 2468.966 kb on + strand at 2468.967 kb on - strand at 2468.967 kb on - strand at 2468.967 kb on - strand at 2468.967 kb on - strand at 2468.967 kb on - strand at 2468.967 kb on - strand at 2468.967 kb on - strand at 2468.971 kb on - strand at 2468.971 kb on - strand at 2468.971 kb on - strand at 2468.971 kb on - strand at 2468.971 kb on - strand at 2468.971 kb on - strand at 2468.971 kb on - strand at 2468.975 kb on + strand at 2468.975 kb on + strand at 2468.975 kb on + strand at 2468.976 kb on - strand at 2468.997 kb on + strand at 2468.998 kb on - strand at 2468.998 kb on - strand at 2468.998 kb on - strand at 2468.998 kb on - strand at 2468.998 kb on - strand at 2468.998 kb on - strand at 2468.998 kb on - strand at 2468.998 kb on - strand at 2469.055 kb on - strand at 2469.055 kb on - strand at 2469.055 kb on - strand at 2469.058 kb on + strand at 2469.093 kb on + strand at 2469.093 kb on + strand at 2469.097 kb on - strand at 2469.097 kb on - strand at 2469.099 kb on - strand at 2469.099 kb on - strand at 2469.223 kb on + strand, within Psest_2317 at 2469.224 kb on - strand, within Psest_2317 at 2469.224 kb on - strand, within Psest_2317 at 2469.224 kb on - strand, within Psest_2317 at 2469.249 kb on + strand, within Psest_2317 at 2469.249 kb on + strand, within Psest_2317 at 2469.253 kb on + strand, within Psest_2317 at 2469.253 kb on + strand, within Psest_2317 at 2469.253 kb on + strand, within Psest_2317 at 2469.253 kb on + strand, within Psest_2317 at 2469.253 kb on + strand, within Psest_2317 at 2469.253 kb on + strand, within Psest_2317 at 2469.254 kb on - strand, within Psest_2317 at 2469.254 kb on - strand, within Psest_2317 at 2469.296 kb on + strand, within Psest_2317 at 2469.453 kb on + strand, within Psest_2317 at 2469.464 kb on + strand, within Psest_2317 at 2469.464 kb on + strand, within Psest_2317 at 2469.495 kb on + strand, within Psest_2317 at 2469.495 kb on + strand, within Psest_2317 at 2469.495 kb on + strand, within Psest_2317 at 2469.495 kb on + strand, within Psest_2317 at 2469.495 kb on + strand, within Psest_2317 at 2469.495 kb on + strand, within Psest_2317 at 2469.495 kb on + strand, within Psest_2317 at 2469.496 kb on - strand, within Psest_2317 at 2469.496 kb on - strand, within Psest_2317 at 2469.496 kb on - strand, within Psest_2317 at 2469.561 kb on + strand, within Psest_2317 at 2469.561 kb on + strand, within Psest_2317 at 2469.614 kb on + strand, within Psest_2317 at 2469.615 kb on - strand, within Psest_2317 at 2469.615 kb on - strand, within Psest_2317 at 2469.624 kb on + strand, within Psest_2317 at 2469.624 kb on + strand, within Psest_2317 at 2469.624 kb on + strand, within Psest_2317 at 2469.625 kb on - strand, within Psest_2317 at 2469.660 kb on + strand, within Psest_2317 at 2469.661 kb on - strand, within Psest_2317 at 2469.717 kb on + strand, within Psest_2317 at 2469.717 kb on + strand, within Psest_2317 at 2469.717 kb on + strand, within Psest_2317 at 2469.717 kb on + strand, within Psest_2317 at 2469.718 kb on - strand, within Psest_2317 at 2469.871 kb on - strand, within Psest_2317 at 2469.952 kb on - strand at 2469.966 kb on + strand at 2470.013 kb on + strand at 2470.014 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Phosphomycin 0.125 mg/ml remove 2,468,190 + Psest_2316 0.67 -0.5 2,468,190 + Psest_2316 0.67 -1.0 2,468,191 - Psest_2316 0.67 -3.1 2,468,271 + Psest_2316 0.70 -4.8 2,468,272 - Psest_2316 0.70 -3.3 2,468,966 + -3.7 2,468,966 + -1.3 2,468,967 - -2.8 2,468,967 - -3.2 2,468,967 - -2.7 2,468,967 - -2.6 2,468,967 - -0.3 2,468,967 - -2.9 2,468,967 - -1.9 2,468,971 - -0.1 2,468,971 - -3.1 2,468,971 - -3.2 2,468,971 - -2.7 2,468,971 - +0.1 2,468,971 - -1.9 2,468,971 - -2.5 2,468,975 + -3.7 2,468,975 + -3.3 2,468,975 + -2.0 2,468,976 - -0.9 2,468,997 + -3.0 2,468,998 - -1.8 2,468,998 - +0.6 2,468,998 - -0.2 2,468,998 - -0.2 2,468,998 - -1.9 2,468,998 - -0.0 2,468,998 - -3.3 2,468,998 - +1.1 2,469,055 - -0.1 2,469,055 - -0.5 2,469,055 - -0.6 2,469,058 + -0.9 2,469,093 + -2.1 2,469,093 + -1.6 2,469,097 - +0.6 2,469,097 - +0.3 2,469,099 - -0.1 2,469,099 - +1.8 2,469,223 + Psest_2317 0.11 -1.2 2,469,224 - Psest_2317 0.11 +0.5 2,469,224 - Psest_2317 0.11 -1.1 2,469,224 - Psest_2317 0.11 -0.7 2,469,249 + Psest_2317 0.14 -2.2 2,469,249 + Psest_2317 0.14 -2.0 2,469,253 + Psest_2317 0.14 -2.5 2,469,253 + Psest_2317 0.14 -1.8 2,469,253 + Psest_2317 0.14 -1.7 2,469,253 + Psest_2317 0.14 -2.9 2,469,253 + Psest_2317 0.14 -1.6 2,469,253 + Psest_2317 0.14 -3.2 2,469,254 - Psest_2317 0.15 +0.7 2,469,254 - Psest_2317 0.15 -1.3 2,469,296 + Psest_2317 0.20 -1.7 2,469,453 + Psest_2317 0.39 -0.9 2,469,464 + Psest_2317 0.40 -1.8 2,469,464 + Psest_2317 0.40 -3.3 2,469,495 + Psest_2317 0.44 -2.2 2,469,495 + Psest_2317 0.44 -1.4 2,469,495 + Psest_2317 0.44 -2.7 2,469,495 + Psest_2317 0.44 -1.9 2,469,495 + Psest_2317 0.44 -2.5 2,469,495 + Psest_2317 0.44 -2.1 2,469,495 + Psest_2317 0.44 -3.3 2,469,496 - Psest_2317 0.44 +0.2 2,469,496 - Psest_2317 0.44 +0.3 2,469,496 - Psest_2317 0.44 +0.7 2,469,561 + Psest_2317 0.52 -2.2 2,469,561 + Psest_2317 0.52 -1.3 2,469,614 + Psest_2317 0.58 -1.8 2,469,615 - Psest_2317 0.59 +1.3 2,469,615 - Psest_2317 0.59 -0.9 2,469,624 + Psest_2317 0.60 -1.3 2,469,624 + Psest_2317 0.60 -2.8 2,469,624 + Psest_2317 0.60 -0.9 2,469,625 - Psest_2317 0.60 +0.6 2,469,660 + Psest_2317 0.64 -1.2 2,469,661 - Psest_2317 0.64 +0.2 2,469,717 + Psest_2317 0.71 -3.1 2,469,717 + Psest_2317 0.71 -2.4 2,469,717 + Psest_2317 0.71 -2.2 2,469,717 + Psest_2317 0.71 +0.3 2,469,718 - Psest_2317 0.71 +1.4 2,469,871 - Psest_2317 0.90 +0.1 2,469,952 - +0.6 2,469,966 + -2.7 2,470,013 + -1.2 2,470,014 - -0.4
Or see this region's nucleotide sequence