Experiment: Def Phosphomycin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1973 and Psest_1974 are separated by 3 nucleotides Psest_1974 and Psest_1975 overlap by 20 nucleotides Psest_1975 and Psest_1976 are separated by 85 nucleotides Psest_1976 and Psest_1978 are separated by 442 nucleotides
Psest_1973: Psest_1973 - phenylalanyl-tRNA synthetase, beta subunit, non-spirochete bacterial, at 2,085,991 to 2,088,369
_1973
Psest_1974: Psest_1974 - integration host factor, alpha subunit, at 2,088,373 to 2,088,675
_1974
Psest_1975: Psest_1975 - Predicted transcriptional regulators, at 2,088,656 to 2,089,012
_1975
Psest_1976: Psest_1976 - tRNA_Pro_GGG, at 2,089,098 to 2,089,174
_1976
Psest_1978: Psest_1978 - DNA-directed DNA polymerase III (polc), at 2,089,617 to 2,092,697
_1978
Position (kb)
2088
2089
2090 Strain fitness (log2 ratio)
-1
0
1
2 at 2088.367 kb on + strand at 2088.367 kb on + strand at 2088.368 kb on - strand at 2088.412 kb on + strand, within Psest_1974 at 2088.413 kb on - strand, within Psest_1974 at 2088.413 kb on - strand, within Psest_1974 at 2088.413 kb on - strand, within Psest_1974 at 2088.433 kb on - strand, within Psest_1974 at 2088.433 kb on - strand, within Psest_1974 at 2088.655 kb on + strand at 2088.655 kb on + strand at 2088.655 kb on + strand at 2088.873 kb on - strand, within Psest_1975 at 2088.910 kb on + strand, within Psest_1975 at 2088.910 kb on + strand, within Psest_1975 at 2088.910 kb on + strand, within Psest_1975 at 2088.911 kb on - strand, within Psest_1975 at 2088.911 kb on - strand, within Psest_1975 at 2088.911 kb on - strand, within Psest_1975 at 2088.911 kb on - strand, within Psest_1975 at 2088.911 kb on - strand, within Psest_1975 at 2088.911 kb on - strand, within Psest_1975 at 2088.911 kb on - strand, within Psest_1975 at 2088.911 kb on - strand, within Psest_1975 at 2088.911 kb on - strand, within Psest_1975 at 2088.911 kb on - strand, within Psest_1975 at 2088.911 kb on - strand, within Psest_1975 at 2088.911 kb on - strand, within Psest_1975 at 2088.911 kb on - strand, within Psest_1975 at 2088.911 kb on - strand, within Psest_1975 at 2088.948 kb on - strand, within Psest_1975 at 2088.958 kb on + strand, within Psest_1975 at 2088.959 kb on - strand, within Psest_1975 at 2089.010 kb on + strand at 2089.010 kb on + strand at 2089.010 kb on + strand at 2089.010 kb on + strand at 2089.054 kb on - strand at 2089.058 kb on + strand at 2089.058 kb on + strand at 2089.058 kb on + strand at 2089.058 kb on + strand at 2089.060 kb on + strand at 2089.178 kb on - strand at 2089.521 kb on + strand at 2089.522 kb on - strand at 2089.536 kb on - strand at 2089.567 kb on + strand at 2089.584 kb on + strand at 2089.585 kb on - strand at 2089.962 kb on + strand, within Psest_1978 at 2089.963 kb on - strand, within Psest_1978
Per-strain Table
Position Strand Gene LocusTag Fraction Def Phosphomycin 0.125 mg/ml remove 2,088,367 + +0.1 2,088,367 + +0.1 2,088,368 - -1.2 2,088,412 + Psest_1974 0.13 -0.3 2,088,413 - Psest_1974 0.13 -1.0 2,088,413 - Psest_1974 0.13 -0.2 2,088,413 - Psest_1974 0.13 -1.7 2,088,433 - Psest_1974 0.20 -0.4 2,088,433 - Psest_1974 0.20 -1.0 2,088,655 + -1.4 2,088,655 + +0.1 2,088,655 + -1.0 2,088,873 - Psest_1975 0.61 +0.2 2,088,910 + Psest_1975 0.71 -1.5 2,088,910 + Psest_1975 0.71 +2.4 2,088,910 + Psest_1975 0.71 +0.3 2,088,911 - Psest_1975 0.71 -0.6 2,088,911 - Psest_1975 0.71 -0.5 2,088,911 - Psest_1975 0.71 -1.0 2,088,911 - Psest_1975 0.71 +0.5 2,088,911 - Psest_1975 0.71 -1.0 2,088,911 - Psest_1975 0.71 -0.3 2,088,911 - Psest_1975 0.71 -1.2 2,088,911 - Psest_1975 0.71 -0.1 2,088,911 - Psest_1975 0.71 -1.0 2,088,911 - Psest_1975 0.71 -0.8 2,088,911 - Psest_1975 0.71 +1.1 2,088,911 - Psest_1975 0.71 -1.3 2,088,911 - Psest_1975 0.71 +0.4 2,088,911 - Psest_1975 0.71 +0.3 2,088,948 - Psest_1975 0.82 -0.3 2,088,958 + Psest_1975 0.85 -0.6 2,088,959 - Psest_1975 0.85 +0.8 2,089,010 + +0.4 2,089,010 + -0.1 2,089,010 + -0.4 2,089,010 + +0.0 2,089,054 - +0.6 2,089,058 + +0.6 2,089,058 + +1.4 2,089,058 + +0.1 2,089,058 + -0.9 2,089,060 + +0.5 2,089,178 - -0.5 2,089,521 + -0.3 2,089,522 - +0.4 2,089,536 - +0.8 2,089,567 + +0.6 2,089,584 + +0.8 2,089,585 - +0.4 2,089,962 + Psest_1978 0.11 -0.4 2,089,963 - Psest_1978 0.11 +0.1
Or see this region's nucleotide sequence