Experiment: R2A with 2-pentyl-furan 21.3684 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE51_RS13300 and ABIE51_RS13305 overlap by 7 nucleotides ABIE51_RS13305 and ABIE51_RS13310 overlap by 4 nucleotides ABIE51_RS13310 and ABIE51_RS13315 overlap by 4 nucleotides
ABIE51_RS13300: ABIE51_RS13300 - imidazole glycerol phosphate synthase subunit HisF, at 2,871,091 to 2,871,867
_RS13300
ABIE51_RS13305: ABIE51_RS13305 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 2,871,861 to 2,872,637
_RS13305
ABIE51_RS13310: ABIE51_RS13310 - imidazole glycerol phosphate synthase subunit HisH, at 2,872,634 to 2,873,236
_RS13310
ABIE51_RS13315: ABIE51_RS13315 - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 2,873,233 to 2,874,309
_RS13315
Position (kb)
2872
2873
2874 Strain fitness (log2 ratio)
-1
0
1 at 2871.847 kb on + strand at 2872.487 kb on - strand, within ABIE51_RS13305 at 2872.759 kb on - strand, within ABIE51_RS13310 at 2872.815 kb on + strand, within ABIE51_RS13310 at 2872.984 kb on - strand, within ABIE51_RS13310 at 2873.042 kb on - strand, within ABIE51_RS13310 at 2873.468 kb on + strand, within ABIE51_RS13315 at 2873.468 kb on + strand, within ABIE51_RS13315
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 2-pentyl-furan 21.3684 mM remove 2,871,847 + -0.1 2,872,487 - ABIE51_RS13305 0.81 -0.0 2,872,759 - ABIE51_RS13310 0.21 +0.5 2,872,815 + ABIE51_RS13310 0.30 -0.1 2,872,984 - ABIE51_RS13310 0.58 -0.1 2,873,042 - ABIE51_RS13310 0.68 +0.0 2,873,468 + ABIE51_RS13315 0.22 +1.3 2,873,468 + ABIE51_RS13315 0.22 -0.4
Or see this region's nucleotide sequence