Experiment: R2A with 1-octen-3-ol 1.08545883333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE51_RS12675 and ABIE51_RS12680 are separated by 10 nucleotides ABIE51_RS12680 and ABIE51_RS12685 are separated by 4 nucleotides
ABIE51_RS12675: ABIE51_RS12675 - amidohydrolase family protein, at 2,733,930 to 2,734,940
_RS12675
ABIE51_RS12680: ABIE51_RS12680 - 5-carboxymethyl-2-hydroxymuconate Delta-isomerase, at 2,734,951 to 2,735,319
_RS12680
ABIE51_RS12685: ABIE51_RS12685 - kynureninase, at 2,735,324 to 2,736,622
_RS12685
Position (kb)
2734
2735
2736 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2733.958 kb on - strand at 2733.958 kb on - strand at 2733.958 kb on - strand at 2733.958 kb on - strand at 2733.958 kb on - strand at 2733.958 kb on - strand at 2734.051 kb on - strand, within ABIE51_RS12675 at 2734.051 kb on - strand, within ABIE51_RS12675 at 2734.051 kb on - strand, within ABIE51_RS12675 at 2734.109 kb on + strand, within ABIE51_RS12675 at 2734.109 kb on + strand, within ABIE51_RS12675 at 2734.109 kb on + strand, within ABIE51_RS12675 at 2734.119 kb on + strand, within ABIE51_RS12675 at 2734.120 kb on - strand, within ABIE51_RS12675 at 2734.120 kb on - strand, within ABIE51_RS12675 at 2734.120 kb on - strand, within ABIE51_RS12675 at 2734.135 kb on + strand, within ABIE51_RS12675 at 2734.135 kb on + strand, within ABIE51_RS12675 at 2734.551 kb on - strand, within ABIE51_RS12675 at 2734.623 kb on + strand, within ABIE51_RS12675 at 2734.623 kb on + strand, within ABIE51_RS12675 at 2734.704 kb on + strand, within ABIE51_RS12675 at 2734.704 kb on + strand, within ABIE51_RS12675 at 2734.704 kb on + strand, within ABIE51_RS12675 at 2734.704 kb on + strand, within ABIE51_RS12675 at 2734.704 kb on + strand, within ABIE51_RS12675 at 2734.705 kb on - strand, within ABIE51_RS12675 at 2734.887 kb on + strand at 2734.888 kb on - strand at 2734.973 kb on - strand at 2734.973 kb on - strand at 2734.973 kb on - strand at 2735.284 kb on + strand at 2735.285 kb on - strand at 2735.285 kb on - strand at 2735.349 kb on - strand at 2735.357 kb on - strand at 2735.360 kb on + strand at 2735.360 kb on + strand at 2735.360 kb on + strand at 2735.360 kb on + strand at 2735.361 kb on - strand at 2735.361 kb on - strand at 2735.361 kb on - strand at 2735.361 kb on - strand at 2735.361 kb on - strand at 2735.400 kb on + strand at 2735.401 kb on - strand at 2735.447 kb on + strand at 2735.447 kb on + strand at 2735.448 kb on - strand at 2735.448 kb on - strand at 2736.062 kb on + strand, within ABIE51_RS12685 at 2736.062 kb on + strand, within ABIE51_RS12685 at 2736.068 kb on + strand, within ABIE51_RS12685 at 2736.069 kb on - strand, within ABIE51_RS12685
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 1.08545883333333 mM remove 2,733,958 - +0.4 2,733,958 - +0.6 2,733,958 - +0.0 2,733,958 - +0.4 2,733,958 - +0.5 2,733,958 - +0.7 2,734,051 - ABIE51_RS12675 0.12 +0.4 2,734,051 - ABIE51_RS12675 0.12 +0.6 2,734,051 - ABIE51_RS12675 0.12 +0.2 2,734,109 + ABIE51_RS12675 0.18 +0.8 2,734,109 + ABIE51_RS12675 0.18 +0.8 2,734,109 + ABIE51_RS12675 0.18 +0.8 2,734,119 + ABIE51_RS12675 0.19 +1.0 2,734,120 - ABIE51_RS12675 0.19 +0.6 2,734,120 - ABIE51_RS12675 0.19 +0.5 2,734,120 - ABIE51_RS12675 0.19 +0.5 2,734,135 + ABIE51_RS12675 0.20 +0.9 2,734,135 + ABIE51_RS12675 0.20 +1.1 2,734,551 - ABIE51_RS12675 0.61 +0.3 2,734,623 + ABIE51_RS12675 0.69 +0.8 2,734,623 + ABIE51_RS12675 0.69 +1.3 2,734,704 + ABIE51_RS12675 0.77 +0.7 2,734,704 + ABIE51_RS12675 0.77 +1.1 2,734,704 + ABIE51_RS12675 0.77 +1.4 2,734,704 + ABIE51_RS12675 0.77 +2.1 2,734,704 + ABIE51_RS12675 0.77 -1.0 2,734,705 - ABIE51_RS12675 0.77 +0.3 2,734,887 + +1.5 2,734,888 - +1.5 2,734,973 - -0.4 2,734,973 - +0.3 2,734,973 - -0.5 2,735,284 + +0.3 2,735,285 - -0.4 2,735,285 - -1.7 2,735,349 - -2.3 2,735,357 - -2.5 2,735,360 + -1.5 2,735,360 + -1.4 2,735,360 + -1.2 2,735,360 + -0.9 2,735,361 - -1.5 2,735,361 - -2.6 2,735,361 - -2.3 2,735,361 - -1.5 2,735,361 - -1.6 2,735,400 + +0.1 2,735,401 - +0.5 2,735,447 + +0.3 2,735,447 + -1.1 2,735,448 - +0.3 2,735,448 - -0.7 2,736,062 + ABIE51_RS12685 0.57 -1.8 2,736,062 + ABIE51_RS12685 0.57 -1.9 2,736,068 + ABIE51_RS12685 0.57 -1.5 2,736,069 - ABIE51_RS12685 0.57 -1.8
Or see this region's nucleotide sequence