Experiment: R2A with 1-octen-3-ol 1.3568235 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE51_RS06735 and ABIE51_RS06740 are separated by 11 nucleotides ABIE51_RS06740 and ABIE51_RS06745 are separated by 39 nucleotides ABIE51_RS06745 and ABIE51_RS06750 are separated by 97 nucleotides
ABIE51_RS06735: ABIE51_RS06735 - DNA polymerase III subunit delta, at 1,439,988 to 1,441,055
_RS06735
ABIE51_RS06740: ABIE51_RS06740 - nicotinate-nucleotide adenylyltransferase, at 1,441,067 to 1,441,744
_RS06740
ABIE51_RS06745: ABIE51_RS06745 - ribosome silencing factor, at 1,441,784 to 1,442,212
_RS06745
ABIE51_RS06750: ABIE51_RS06750 - alkaline phosphatase PhoX, at 1,442,310 to 1,443,860
_RS06750
Position (kb)
1441
1442
1443 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1441.082 kb on + strand at 1441.082 kb on + strand at 1441.709 kb on + strand at 1441.710 kb on - strand at 1441.740 kb on + strand at 1441.740 kb on + strand at 1441.740 kb on + strand at 1441.740 kb on + strand at 1441.740 kb on + strand at 1441.741 kb on - strand at 1441.743 kb on - strand at 1441.789 kb on + strand at 1441.790 kb on - strand at 1441.790 kb on - strand at 1441.937 kb on + strand, within ABIE51_RS06745 at 1441.937 kb on + strand, within ABIE51_RS06745 at 1441.937 kb on + strand, within ABIE51_RS06745 at 1441.938 kb on - strand, within ABIE51_RS06745 at 1441.938 kb on - strand, within ABIE51_RS06745 at 1441.938 kb on - strand, within ABIE51_RS06745 at 1442.124 kb on - strand, within ABIE51_RS06745 at 1442.124 kb on - strand, within ABIE51_RS06745 at 1442.351 kb on + strand at 1442.352 kb on - strand at 1442.432 kb on - strand at 1442.432 kb on - strand at 1442.506 kb on + strand, within ABIE51_RS06750 at 1442.507 kb on - strand, within ABIE51_RS06750 at 1442.557 kb on + strand, within ABIE51_RS06750 at 1442.558 kb on - strand, within ABIE51_RS06750 at 1442.779 kb on + strand, within ABIE51_RS06750 at 1442.779 kb on + strand, within ABIE51_RS06750 at 1442.779 kb on + strand, within ABIE51_RS06750 at 1442.780 kb on - strand, within ABIE51_RS06750 at 1442.780 kb on - strand, within ABIE51_RS06750 at 1442.780 kb on - strand, within ABIE51_RS06750 at 1442.873 kb on + strand, within ABIE51_RS06750 at 1442.874 kb on - strand, within ABIE51_RS06750 at 1442.945 kb on - strand, within ABIE51_RS06750 at 1442.945 kb on - strand, within ABIE51_RS06750 at 1442.945 kb on - strand, within ABIE51_RS06750 at 1442.945 kb on - strand, within ABIE51_RS06750 at 1443.037 kb on + strand, within ABIE51_RS06750 at 1443.070 kb on + strand, within ABIE51_RS06750 at 1443.106 kb on + strand, within ABIE51_RS06750 at 1443.106 kb on + strand, within ABIE51_RS06750 at 1443.107 kb on - strand, within ABIE51_RS06750 at 1443.107 kb on - strand, within ABIE51_RS06750 at 1443.107 kb on - strand, within ABIE51_RS06750
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 1.3568235 mM remove 1,441,082 + -1.3 1,441,082 + -3.0 1,441,709 + -0.1 1,441,710 - -0.6 1,441,740 + +0.9 1,441,740 + -0.2 1,441,740 + -0.1 1,441,740 + +0.2 1,441,740 + +0.3 1,441,741 - -0.5 1,441,743 - -0.7 1,441,789 + -0.4 1,441,790 - -0.2 1,441,790 - -0.2 1,441,937 + ABIE51_RS06745 0.36 -0.8 1,441,937 + ABIE51_RS06745 0.36 -0.7 1,441,937 + ABIE51_RS06745 0.36 -0.2 1,441,938 - ABIE51_RS06745 0.36 -0.1 1,441,938 - ABIE51_RS06745 0.36 -0.0 1,441,938 - ABIE51_RS06745 0.36 -0.0 1,442,124 - ABIE51_RS06745 0.79 -0.6 1,442,124 - ABIE51_RS06745 0.79 +0.1 1,442,351 + -0.6 1,442,352 - +0.4 1,442,432 - -0.3 1,442,432 - -0.1 1,442,506 + ABIE51_RS06750 0.13 +0.1 1,442,507 - ABIE51_RS06750 0.13 +1.1 1,442,557 + ABIE51_RS06750 0.16 +0.2 1,442,558 - ABIE51_RS06750 0.16 +0.3 1,442,779 + ABIE51_RS06750 0.30 -0.0 1,442,779 + ABIE51_RS06750 0.30 -0.1 1,442,779 + ABIE51_RS06750 0.30 -0.9 1,442,780 - ABIE51_RS06750 0.30 +1.5 1,442,780 - ABIE51_RS06750 0.30 +0.3 1,442,780 - ABIE51_RS06750 0.30 -0.8 1,442,873 + ABIE51_RS06750 0.36 -0.1 1,442,874 - ABIE51_RS06750 0.36 +0.3 1,442,945 - ABIE51_RS06750 0.41 -0.2 1,442,945 - ABIE51_RS06750 0.41 -0.8 1,442,945 - ABIE51_RS06750 0.41 +0.4 1,442,945 - ABIE51_RS06750 0.41 -0.2 1,443,037 + ABIE51_RS06750 0.47 -1.0 1,443,070 + ABIE51_RS06750 0.49 -0.8 1,443,106 + ABIE51_RS06750 0.51 +0.3 1,443,106 + ABIE51_RS06750 0.51 -0.0 1,443,107 - ABIE51_RS06750 0.51 -0.1 1,443,107 - ABIE51_RS06750 0.51 -0.2 1,443,107 - ABIE51_RS06750 0.51 +0.1
Or see this region's nucleotide sequence