Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3649

Experiment: 7H9 with Hydrogen peroxide 2.5 mM; pH 6.5

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3647c and Rv3648c are separated by 139 nucleotidesRv3648c and Rv3649 are separated by 249 nucleotidesRv3649 and Rv3650 are separated by 136 nucleotidesRv3650 and Rv3651 are separated by 323 nucleotides Rv3647c: Rv3647c - Conserved hypothetical protein, at 4,087,610 to 4,088,188 Rv3647c Rv3648c: Rv3648c - Probable cold shock protein A CspA, at 4,088,328 to 4,088,531 Rv3648c Rv3649: Rv3649 - Probable helicase, at 4,088,781 to 4,091,096 Rv3649 Rv3650: Rv3650 - PE family protein PE33, at 4,091,233 to 4,091,517 Rv3650 Rv3651: Rv3651 - Conserved hypothetical protein, at 4,091,841 to 4,092,878 Rv3651 Position (kb) 4088 4089 4090 4091 4092Strain fitness (log2 ratio) -2 -1 0 1 2at 4088.070 kb on - strand, within Rv3647cat 4088.154 kb on - strandat 4088.154 kb on - strandat 4088.227 kb on - strandat 4088.270 kb on + strandat 4088.270 kb on + strandat 4088.271 kb on - strandat 4088.271 kb on - strandat 4088.766 kb on - strandat 4088.766 kb on - strandat 4088.991 kb on + strandat 4089.202 kb on - strand, within Rv3649at 4089.286 kb on + strand, within Rv3649at 4089.413 kb on - strand, within Rv3649at 4089.789 kb on + strand, within Rv3649at 4089.897 kb on - strand, within Rv3649at 4090.035 kb on + strand, within Rv3649at 4090.036 kb on - strand, within Rv3649at 4090.243 kb on - strand, within Rv3649at 4091.189 kb on - strandat 4091.417 kb on - strand, within Rv3650at 4091.571 kb on + strandat 4091.572 kb on - strandat 4091.605 kb on + strandat 4091.606 kb on - strandat 4092.061 kb on + strand, within Rv3651at 4092.061 kb on + strand, within Rv3651

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Hydrogen peroxide 2.5 mM; pH 6.5
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4,088,070 - Rv3647c 0.79 +1.2
4,088,154 - -0.4
4,088,154 - -0.6
4,088,227 - -0.8
4,088,270 + +0.8
4,088,270 + -0.1
4,088,271 - -0.6
4,088,271 - +0.2
4,088,766 - -0.1
4,088,766 - +0.1
4,088,991 + +0.2
4,089,202 - Rv3649 0.18 -1.1
4,089,286 + Rv3649 0.22 -2.3
4,089,413 - Rv3649 0.27 +0.0
4,089,789 + Rv3649 0.44 -0.9
4,089,897 - Rv3649 0.48 -1.5
4,090,035 + Rv3649 0.54 +0.0
4,090,036 - Rv3649 0.54 -0.1
4,090,243 - Rv3649 0.63 -0.3
4,091,189 - -0.1
4,091,417 - Rv3650 0.65 -0.8
4,091,571 + -0.7
4,091,572 - +1.8
4,091,605 + -0.2
4,091,606 - -0.2
4,092,061 + Rv3651 0.21 -1.2
4,092,061 + Rv3651 0.21 -0.4

Or see this region's nucleotide sequence