Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3319

Experiment: 7H9 with Hydrogen peroxide 2.5 mM; pH 6.5

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3318 and Rv3319 overlap by 1 nucleotidesRv3319 and Rv3320c are separated by 78 nucleotidesRv3320c and Rv3321c are separated by 3 nucleotidesRv3321c and Rv3322c are separated by 121 nucleotides Rv3318: Rv3318 - Probable succinate dehydrogenase (flavoprotein subunit) SdhA (succinic dehydrogenase) (fumarate reductase) (fumarate dehydrogenase) (fumaric hydrogenase), at 3,705,000 to 3,706,772 Rv3318 Rv3319: Rv3319 - Probable succinate dehydrogenase (iron-sulphur protein subunit) SdhB (succinic dehydrogenase) (fumarate reductase) (fumarate dehydrogenase) (fumaric hydrogenase), at 3,706,772 to 3,707,563 Rv3319 Rv3320c: Rv3320c - Possible toxin VapC44. Contains PIN domain., at 3,707,642 to 3,708,070 Rv3320c Rv3321c: Rv3321c - Possible antitoxin VapB44, at 3,708,074 to 3,708,316 Rv3321c Rv3322c: Rv3322c - Possible methyltransferase, at 3,708,438 to 3,709,052 Rv3322c Position (kb) 3706 3707 3708Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3705.927 kb on + strand, within Rv3318at 3705.928 kb on - strand, within Rv3318at 3705.928 kb on - strand, within Rv3318at 3705.934 kb on - strand, within Rv3318at 3706.062 kb on + strand, within Rv3318at 3706.208 kb on + strand, within Rv3318at 3706.252 kb on - strand, within Rv3318at 3706.618 kb on - strandat 3706.767 kb on + strandat 3706.770 kb on + strandat 3706.771 kb on - strandat 3706.961 kb on + strand, within Rv3319at 3706.962 kb on - strand, within Rv3319at 3706.962 kb on - strand, within Rv3319at 3706.974 kb on - strand, within Rv3319at 3706.974 kb on - strand, within Rv3319at 3707.195 kb on + strand, within Rv3319at 3707.213 kb on + strand, within Rv3319at 3707.280 kb on - strand, within Rv3319at 3707.354 kb on + strand, within Rv3319at 3707.751 kb on + strand, within Rv3320cat 3708.204 kb on - strand, within Rv3321cat 3708.284 kb on + strand, within Rv3321cat 3708.285 kb on - strand, within Rv3321cat 3708.439 kb on + strandat 3708.439 kb on + strandat 3708.440 kb on - strandat 3708.440 kb on - strandat 3708.440 kb on - strandat 3708.440 kb on - strandat 3708.440 kb on - strandat 3708.440 kb on - strandat 3708.440 kb on - strandat 3708.440 kb on - strandat 3708.501 kb on - strand, within Rv3322cat 3708.516 kb on + strand, within Rv3322cat 3708.516 kb on + strand, within Rv3322c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Hydrogen peroxide 2.5 mM; pH 6.5
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3,705,927 + Rv3318 0.52 +1.3
3,705,928 - Rv3318 0.52 -0.4
3,705,928 - Rv3318 0.52 +0.3
3,705,934 - Rv3318 0.53 -1.0
3,706,062 + Rv3318 0.60 -3.0
3,706,208 + Rv3318 0.68 +1.8
3,706,252 - Rv3318 0.71 -0.7
3,706,618 - -0.4
3,706,767 + -3.5
3,706,770 + +1.8
3,706,771 - -2.2
3,706,961 + Rv3319 0.24 -0.6
3,706,962 - Rv3319 0.24 +0.3
3,706,962 - Rv3319 0.24 -0.9
3,706,974 - Rv3319 0.26 -1.3
3,706,974 - Rv3319 0.26 -0.6
3,707,195 + Rv3319 0.53 -1.0
3,707,213 + Rv3319 0.56 -1.4
3,707,280 - Rv3319 0.64 -0.2
3,707,354 + Rv3319 0.73 -0.3
3,707,751 + Rv3320c 0.25 -0.8
3,708,204 - Rv3321c 0.53 -1.0
3,708,284 + Rv3321c 0.86 -0.3
3,708,285 - Rv3321c 0.87 -0.5
3,708,439 + -0.8
3,708,439 + -0.8
3,708,440 - +0.0
3,708,440 - +0.3
3,708,440 - +0.0
3,708,440 - -2.2
3,708,440 - +0.8
3,708,440 - -0.6
3,708,440 - -1.9
3,708,440 - -0.7
3,708,501 - Rv3322c 0.10 +1.2
3,708,516 + Rv3322c 0.13 -0.5
3,708,516 + Rv3322c 0.13 -0.0

Or see this region's nucleotide sequence