Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3175

Experiment: 7H9 with Hydrogen peroxide 2.5 mM; pH 6.5

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3173c and Rv3174 are separated by 92 nucleotidesRv3174 and Rv3175 are separated by 14 nucleotidesRv3175 and Rv3176c overlap by 4 nucleotides Rv3173c: Rv3173c - Probable transcriptional regulatory protein (probably TetR/AcrR-family), at 3,541,443 to 3,542,045 Rv3173c Rv3174: Rv3174 - Probable short-chain dehydrogenase/reductase, at 3,542,138 to 3,542,845 Rv3174 Rv3175: Rv3175 - Possible amidase (aminohydrolase), at 3,542,860 to 3,544,347 Rv3175 Rv3176c: Rv3176c - Probable epoxide hydrolase MesT (epoxide hydratase) (arene-oxide hydratase), at 3,544,344 to 3,545,300 Rv3176c Position (kb) 3542 3543 3544 3545Strain fitness (log2 ratio) -1 0 1 2at 3541.866 kb on - strand, within Rv3173cat 3541.983 kb on - strand, within Rv3173cat 3541.983 kb on - strand, within Rv3173cat 3542.045 kb on + strandat 3542.069 kb on + strandat 3542.091 kb on - strandat 3542.202 kb on - strandat 3542.561 kb on + strand, within Rv3174at 3542.649 kb on - strand, within Rv3174at 3542.839 kb on + strandat 3542.990 kb on - strandat 3543.127 kb on + strand, within Rv3175at 3543.127 kb on + strand, within Rv3175at 3543.127 kb on + strand, within Rv3175at 3543.127 kb on + strand, within Rv3175at 3543.178 kb on + strand, within Rv3175at 3543.178 kb on + strand, within Rv3175at 3543.178 kb on + strand, within Rv3175at 3543.179 kb on - strand, within Rv3175at 3543.182 kb on + strand, within Rv3175at 3543.183 kb on - strand, within Rv3175at 3543.183 kb on - strand, within Rv3175at 3543.395 kb on - strand, within Rv3175at 3543.635 kb on - strand, within Rv3175at 3543.963 kb on - strand, within Rv3175at 3544.151 kb on - strand, within Rv3175at 3544.230 kb on + strandat 3544.289 kb on + strandat 3544.289 kb on + strandat 3544.290 kb on - strandat 3544.346 kb on - strandat 3544.346 kb on - strandat 3544.371 kb on - strandat 3544.927 kb on + strand, within Rv3176cat 3545.017 kb on + strand, within Rv3176cat 3545.054 kb on - strand, within Rv3176cat 3545.063 kb on - strand, within Rv3176cat 3545.119 kb on + strand, within Rv3176cat 3545.119 kb on + strand, within Rv3176cat 3545.181 kb on + strand, within Rv3176cat 3545.181 kb on + strand, within Rv3176cat 3545.181 kb on + strand, within Rv3176c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Hydrogen peroxide 2.5 mM; pH 6.5
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3,541,866 - Rv3173c 0.70 -0.1
3,541,983 - Rv3173c 0.90 -0.6
3,541,983 - Rv3173c 0.90 -0.8
3,542,045 + -0.4
3,542,069 + +1.5
3,542,091 - -0.1
3,542,202 - +0.4
3,542,561 + Rv3174 0.60 -0.4
3,542,649 - Rv3174 0.72 +0.2
3,542,839 + +0.4
3,542,990 - -0.2
3,543,127 + Rv3175 0.18 +0.1
3,543,127 + Rv3175 0.18 +0.3
3,543,127 + Rv3175 0.18 +0.2
3,543,127 + Rv3175 0.18 +1.3
3,543,178 + Rv3175 0.21 +0.0
3,543,178 + Rv3175 0.21 -0.5
3,543,178 + Rv3175 0.21 +0.9
3,543,179 - Rv3175 0.21 +0.0
3,543,182 + Rv3175 0.22 -0.1
3,543,183 - Rv3175 0.22 +0.0
3,543,183 - Rv3175 0.22 -0.0
3,543,395 - Rv3175 0.36 -1.0
3,543,635 - Rv3175 0.52 -0.8
3,543,963 - Rv3175 0.74 -0.3
3,544,151 - Rv3175 0.87 +1.8
3,544,230 + -1.0
3,544,289 + -0.6
3,544,289 + +1.3
3,544,290 - -0.2
3,544,346 - +0.1
3,544,346 - -0.5
3,544,371 - -0.7
3,544,927 + Rv3176c 0.61 +0.8
3,545,017 + Rv3176c 0.70 -0.8
3,545,054 - Rv3176c 0.74 +1.8
3,545,063 - Rv3176c 0.75 +0.3
3,545,119 + Rv3176c 0.81 +0.5
3,545,119 + Rv3176c 0.81 +0.6
3,545,181 + Rv3176c 0.87 -1.2
3,545,181 + Rv3176c 0.87 -0.2
3,545,181 + Rv3176c 0.87 +0.1

Or see this region's nucleotide sequence