Experiment: 7H9 with Hydrogen peroxide 2.5 mM; pH 6.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv3083 and Rv3084 are separated by 5 nucleotides Rv3084 and Rv3085 overlap by 4 nucleotides Rv3085 and Rv3086 are separated by 30 nucleotides
Rv3083: Rv3083 - Probable monooxygenase (hydroxylase), at 3,448,504 to 3,449,991
Rv3083
Rv3084: Rv3084 - Probable acetyl-hydrolase/esterase LipR, at 3,449,997 to 3,450,923
Rv3084
Rv3085: Rv3085 - Probable short-chain type dehydrogenase/reductase, at 3,450,920 to 3,451,750
Rv3085
Rv3086: Rv3086 - Probable zinc-type alcohol dehydrogenase AdhD (aldehyde reductase), at 3,451,781 to 3,452,887
Rv3086
Position (kb)
3449
3450
3451 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3449.149 kb on + strand, within Rv3083 at 3449.149 kb on + strand, within Rv3083 at 3449.266 kb on + strand, within Rv3083 at 3449.321 kb on + strand, within Rv3083 at 3449.620 kb on + strand, within Rv3083 at 3449.620 kb on + strand, within Rv3083 at 3449.620 kb on + strand, within Rv3083 at 3449.621 kb on - strand, within Rv3083 at 3449.621 kb on - strand, within Rv3083 at 3449.671 kb on + strand, within Rv3083 at 3449.756 kb on - strand, within Rv3083 at 3449.830 kb on + strand, within Rv3083 at 3450.029 kb on - strand at 3450.029 kb on - strand at 3450.031 kb on + strand at 3450.031 kb on + strand at 3450.319 kb on - strand, within Rv3084 at 3450.619 kb on + strand, within Rv3084 at 3450.620 kb on - strand, within Rv3084 at 3450.620 kb on - strand, within Rv3084 at 3450.647 kb on + strand, within Rv3084 at 3451.181 kb on + strand, within Rv3085 at 3451.203 kb on - strand, within Rv3085 at 3451.697 kb on + strand at 3451.697 kb on + strand at 3451.756 kb on + strand at 3451.756 kb on + strand at 3451.881 kb on - strand at 3451.881 kb on - strand at 3451.881 kb on - strand at 3451.881 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Hydrogen peroxide 2.5 mM; pH 6.5 remove 3,449,149 + Rv3083 0.43 -0.1 3,449,149 + Rv3083 0.43 +1.8 3,449,266 + Rv3083 0.51 -0.4 3,449,321 + Rv3083 0.55 +0.8 3,449,620 + Rv3083 0.75 -0.3 3,449,620 + Rv3083 0.75 +0.1 3,449,620 + Rv3083 0.75 +0.2 3,449,621 - Rv3083 0.75 -0.2 3,449,621 - Rv3083 0.75 -0.2 3,449,671 + Rv3083 0.78 +0.2 3,449,756 - Rv3083 0.84 +0.3 3,449,830 + Rv3083 0.89 -0.7 3,450,029 - -1.8 3,450,029 - +0.0 3,450,031 + -1.5 3,450,031 + -0.5 3,450,319 - Rv3084 0.35 +1.5 3,450,619 + Rv3084 0.67 -0.3 3,450,620 - Rv3084 0.67 +0.2 3,450,620 - Rv3084 0.67 +2.3 3,450,647 + Rv3084 0.70 -0.3 3,451,181 + Rv3085 0.31 -2.2 3,451,203 - Rv3085 0.34 -0.5 3,451,697 + +0.0 3,451,697 + +0.1 3,451,756 + -0.1 3,451,756 + -0.6 3,451,881 - -1.5 3,451,881 - +0.1 3,451,881 - -0.2 3,451,881 - -0.1
Or see this region's nucleotide sequence