Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2389c

Experiment: 7H9 with Hydrogen peroxide 2.5 mM; pH 6.5

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2388c and Rv2389c are separated by 105 nucleotidesRv2389c and Rv2390c overlap by 4 nucleotidesRv2390c and Rv2391 are separated by 412 nucleotides Rv2388c: Rv2388c - Probable oxygen-independent coproporphyrinogen III oxidase HemN (coproporphyrinogenase) (coprogen oxidase), at 2,682,015 to 2,683,142 Rv2388c Rv2389c: Rv2389c - Probable resuscitation-promoting factor RpfD, at 2,683,248 to 2,683,712 Rv2389c Rv2390c: Rv2390c - Conserved hypothetical protein, at 2,683,709 to 2,684,266 Rv2390c Rv2391: Rv2391 - Ferredoxin-dependent sulfite reductase SirA, at 2,684,679 to 2,686,370 Rv2391 Position (kb) 2683 2684Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2682.275 kb on - strand, within Rv2388cat 2682.275 kb on - strand, within Rv2388cat 2682.319 kb on + strand, within Rv2388cat 2682.421 kb on + strand, within Rv2388cat 2682.422 kb on - strand, within Rv2388cat 2682.422 kb on - strand, within Rv2388cat 2682.422 kb on - strand, within Rv2388cat 2682.467 kb on - strand, within Rv2388cat 2682.560 kb on - strand, within Rv2388cat 2682.644 kb on - strand, within Rv2388cat 2682.952 kb on + strand, within Rv2388cat 2682.952 kb on + strand, within Rv2388cat 2682.952 kb on + strand, within Rv2388cat 2682.953 kb on - strand, within Rv2388cat 2682.953 kb on - strand, within Rv2388cat 2682.953 kb on - strand, within Rv2388cat 2683.063 kb on + strandat 2683.064 kb on - strandat 2683.067 kb on + strandat 2683.067 kb on + strandat 2683.067 kb on + strandat 2683.068 kb on - strandat 2683.183 kb on - strandat 2683.210 kb on - strandat 2683.216 kb on + strandat 2683.216 kb on + strandat 2683.216 kb on + strandat 2683.217 kb on - strandat 2683.217 kb on - strandat 2683.340 kb on + strand, within Rv2389cat 2683.340 kb on + strand, within Rv2389cat 2683.341 kb on - strand, within Rv2389cat 2683.349 kb on + strand, within Rv2389cat 2683.349 kb on + strand, within Rv2389cat 2683.349 kb on + strand, within Rv2389cat 2683.350 kb on - strand, within Rv2389cat 2683.385 kb on + strand, within Rv2389cat 2683.385 kb on + strand, within Rv2389cat 2683.385 kb on + strand, within Rv2389cat 2683.385 kb on + strand, within Rv2389cat 2683.386 kb on - strand, within Rv2389cat 2683.386 kb on - strand, within Rv2389cat 2683.386 kb on - strand, within Rv2389cat 2683.489 kb on + strand, within Rv2389cat 2683.490 kb on - strand, within Rv2389cat 2683.492 kb on - strand, within Rv2389cat 2683.647 kb on + strand, within Rv2389cat 2683.648 kb on - strand, within Rv2389cat 2683.648 kb on - strand, within Rv2389cat 2683.648 kb on - strand, within Rv2389cat 2683.691 kb on + strandat 2683.694 kb on + strandat 2683.695 kb on - strandat 2683.695 kb on - strandat 2683.695 kb on - strandat 2683.695 kb on - strandat 2683.695 kb on - strandat 2683.712 kb on + strandat 2683.712 kb on + strandat 2683.712 kb on + strandat 2683.712 kb on + strandat 2683.844 kb on + strand, within Rv2390cat 2683.845 kb on - strand, within Rv2390cat 2683.845 kb on - strand, within Rv2390cat 2683.950 kb on + strand, within Rv2390cat 2683.950 kb on + strand, within Rv2390cat 2683.950 kb on + strand, within Rv2390cat 2683.955 kb on - strand, within Rv2390cat 2684.043 kb on + strand, within Rv2390cat 2684.043 kb on + strand, within Rv2390cat 2684.043 kb on + strand, within Rv2390cat 2684.043 kb on + strand, within Rv2390cat 2684.044 kb on - strand, within Rv2390cat 2684.044 kb on - strand, within Rv2390cat 2684.044 kb on - strand, within Rv2390cat 2684.044 kb on - strand, within Rv2390cat 2684.611 kb on + strandat 2684.611 kb on + strandat 2684.611 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Hydrogen peroxide 2.5 mM; pH 6.5
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2,682,275 - Rv2388c 0.23 -1.1
2,682,275 - Rv2388c 0.23 -0.4
2,682,319 + Rv2388c 0.27 -1.3
2,682,421 + Rv2388c 0.36 -1.8
2,682,422 - Rv2388c 0.36 -0.7
2,682,422 - Rv2388c 0.36 -1.2
2,682,422 - Rv2388c 0.36 -0.6
2,682,467 - Rv2388c 0.40 -0.1
2,682,560 - Rv2388c 0.48 -0.5
2,682,644 - Rv2388c 0.56 +0.1
2,682,952 + Rv2388c 0.83 -0.5
2,682,952 + Rv2388c 0.83 -0.0
2,682,952 + Rv2388c 0.83 -0.4
2,682,953 - Rv2388c 0.83 -0.7
2,682,953 - Rv2388c 0.83 +0.0
2,682,953 - Rv2388c 0.83 -0.5
2,683,063 + -0.2
2,683,064 - -1.9
2,683,067 + -0.3
2,683,067 + +0.5
2,683,067 + -0.7
2,683,068 - +0.7
2,683,183 - -0.3
2,683,210 - +0.1
2,683,216 + -2.1
2,683,216 + -0.1
2,683,216 + -0.3
2,683,217 - +0.1
2,683,217 - +3.5
2,683,340 + Rv2389c 0.20 +0.1
2,683,340 + Rv2389c 0.20 +2.0
2,683,341 - Rv2389c 0.20 +0.1
2,683,349 + Rv2389c 0.22 +0.3
2,683,349 + Rv2389c 0.22 +0.2
2,683,349 + Rv2389c 0.22 +0.4
2,683,350 - Rv2389c 0.22 -0.5
2,683,385 + Rv2389c 0.29 -0.4
2,683,385 + Rv2389c 0.29 -0.1
2,683,385 + Rv2389c 0.29 +0.8
2,683,385 + Rv2389c 0.29 +0.6
2,683,386 - Rv2389c 0.30 -0.2
2,683,386 - Rv2389c 0.30 -0.3
2,683,386 - Rv2389c 0.30 -0.3
2,683,489 + Rv2389c 0.52 +0.7
2,683,490 - Rv2389c 0.52 -0.4
2,683,492 - Rv2389c 0.52 -0.6
2,683,647 + Rv2389c 0.86 +0.5
2,683,648 - Rv2389c 0.86 -0.3
2,683,648 - Rv2389c 0.86 +1.6
2,683,648 - Rv2389c 0.86 +1.4
2,683,691 + -0.2
2,683,694 + -0.3
2,683,695 - +0.7
2,683,695 - +0.2
2,683,695 - -1.3
2,683,695 - +1.4
2,683,695 - +0.3
2,683,712 + -1.4
2,683,712 + +0.7
2,683,712 + -0.3
2,683,712 + +2.1
2,683,844 + Rv2390c 0.24 +0.2
2,683,845 - Rv2390c 0.24 +0.6
2,683,845 - Rv2390c 0.24 -0.9
2,683,950 + Rv2390c 0.43 -0.9
2,683,950 + Rv2390c 0.43 -0.0
2,683,950 + Rv2390c 0.43 +2.7
2,683,955 - Rv2390c 0.44 -2.9
2,684,043 + Rv2390c 0.60 +0.8
2,684,043 + Rv2390c 0.60 +0.5
2,684,043 + Rv2390c 0.60 -0.4
2,684,043 + Rv2390c 0.60 +0.3
2,684,044 - Rv2390c 0.60 -0.7
2,684,044 - Rv2390c 0.60 -1.2
2,684,044 - Rv2390c 0.60 -0.2
2,684,044 - Rv2390c 0.60 -0.2
2,684,611 + +0.1
2,684,611 + -0.9
2,684,611 + -0.3

Or see this region's nucleotide sequence