Experiment: 7H9 with Hydrogen peroxide 2.5 mM; pH 6.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv2272 and Rv2273 overlap by 4 nucleotides Rv2273 and Rv2274c are separated by 56 nucleotides Rv2274c and Rv2274A are separated by 33 nucleotides Rv2274A and Rv2275 overlap by 205 nucleotides Rv2275 and Rv2276 overlap by 4 nucleotides
Rv2272: Rv2272 - Probable conserved transmembrane protein, at 2,545,737 to 2,546,105
Rv2272
Rv2273: Rv2273 - Probable conserved transmembrane protein, at 2,546,102 to 2,546,431
Rv2273
Rv2274c: Rv2274c - Possible toxin MazF8, at 2,546,488 to 2,546,805
Rv2274c
Rv2274A: Rv2274A - Possible antitoxin MazE8, at 2,546,839 to 2,547,087
Rv2274A
Rv2275: Rv2275 - Conserved hypothetical protein, at 2,546,883 to 2,547,752
Rv2275
Rv2276: Rv2276 - Cytochrome P450 121 Cyp121, at 2,547,749 to 2,548,939
Rv2276
Position (kb)
2546
2547
2548 Strain fitness (log2 ratio)
-2
-1
0
1 at 2546.032 kb on - strand, within Rv2272 at 2546.291 kb on + strand, within Rv2273 at 2546.291 kb on + strand, within Rv2273 at 2546.291 kb on + strand, within Rv2273 at 2546.305 kb on - strand, within Rv2273 at 2546.490 kb on - strand at 2546.707 kb on + strand, within Rv2274c at 2546.708 kb on - strand, within Rv2274c at 2546.779 kb on - strand at 2546.800 kb on - strand at 2547.160 kb on - strand, within Rv2275 at 2547.567 kb on + strand, within Rv2275 at 2547.568 kb on - strand, within Rv2275 at 2547.672 kb on + strand at 2548.234 kb on + strand, within Rv2276 at 2548.497 kb on + strand, within Rv2276
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Hydrogen peroxide 2.5 mM; pH 6.5 remove 2,546,032 - Rv2272 0.80 +0.8 2,546,291 + Rv2273 0.57 +0.2 2,546,291 + Rv2273 0.57 -0.1 2,546,291 + Rv2273 0.57 -0.3 2,546,305 - Rv2273 0.62 -0.1 2,546,490 - -0.3 2,546,707 + Rv2274c 0.69 -0.4 2,546,708 - Rv2274c 0.69 +0.9 2,546,779 - -0.1 2,546,800 - -1.1 2,547,160 - Rv2275 0.32 -2.4 2,547,567 + Rv2275 0.79 -1.1 2,547,568 - Rv2275 0.79 -0.3 2,547,672 + -2.2 2,548,234 + Rv2276 0.41 +0.8 2,548,497 + Rv2276 0.63 -2.3
Or see this region's nucleotide sequence