Experiment: 7H9 with Hydrogen peroxide 2.5 mM; pH 6.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0538 and Rv0539 are separated by 56 nucleotides Rv0539 and Rv0540 overlap by 4 nucleotides Rv0540 and Rv0541c are separated by 20 nucleotides
Rv0538: Rv0538 - Possible conserved membrane protein, at 630,040 to 631,686
Rv0538
Rv0539: Rv0539 - Probable dolichyl-phosphate sugar synthase (dolichol-phosphate sugar synthetase) (dolichol-phosphate sugar transferase) (sugar phosphoryldolichol synthase), at 631,743 to 632,375
Rv0539
Rv0540: Rv0540 - Conserved hypothetical protein, at 632,372 to 633,034
Rv0540
Rv0541c: Rv0541c - Probable conserved integral membrane protein, at 633,055 to 634,404
Rv0541c
Position (kb) 631
632
633 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 630.766 kb on + strand, within Rv0538 at 630.767 kb on - strand, within Rv0538 at 630.784 kb on + strand, within Rv0538 at 630.784 kb on + strand, within Rv0538 at 630.826 kb on + strand, within Rv0538 at 630.827 kb on - strand, within Rv0538 at 631.670 kb on + strand at 631.670 kb on + strand at 631.670 kb on + strand at 631.670 kb on + strand at 631.670 kb on + strand at 632.283 kb on + strand, within Rv0539 at 632.284 kb on - strand, within Rv0539 at 632.552 kb on - strand, within Rv0540 at 632.602 kb on + strand, within Rv0540 at 632.603 kb on - strand, within Rv0540 at 632.827 kb on + strand, within Rv0540 at 632.827 kb on + strand, within Rv0540 at 633.057 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Hydrogen peroxide 2.5 mM; pH 6.5 remove 630,766 + Rv0538 0.44 -2.4 630,767 - Rv0538 0.44 -0.3 630,784 + Rv0538 0.45 -0.7 630,784 + Rv0538 0.45 -0.5 630,826 + Rv0538 0.48 -0.1 630,827 - Rv0538 0.48 +1.4 631,670 + -0.6 631,670 + +0.3 631,670 + +3.1 631,670 + +0.7 631,670 + +0.6 632,283 + Rv0539 0.85 -1.2 632,284 - Rv0539 0.85 -0.8 632,552 - Rv0540 0.27 -1.2 632,602 + Rv0540 0.35 +1.2 632,603 - Rv0540 0.35 +1.5 632,827 + Rv0540 0.69 +1.6 632,827 + Rv0540 0.69 +0.0 633,057 - +4.1
Or see this region's nucleotide sequence