Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0068

Experiment: 7H9 with Hydrogen peroxide 2.5 mM; pH 6.5

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0066c and Rv0067c are separated by 117 nucleotidesRv0067c and Rv0068 are separated by 102 nucleotidesRv0068 and Rv0069c are separated by 24 nucleotides Rv0066c: Rv0066c - Probable isocitrate dehydrogenase [NADP] Icd2 (oxalosuccinate decarboxylase) (IDH) (NADP+-specific ICDH) (IDP), at 72,274 to 74,511 Rv0066c Rv0067c: Rv0067c - Possible transcriptional regulatory protein (possibly TetR-family), at 74,629 to 75,198 Rv0067c Rv0068: Rv0068 - Probable oxidoreductase, at 75,301 to 76,212 Rv0068 Rv0069c: Rv0069c - Probable L-serine dehydratase SdaA (L-serine deaminase) (SDH) (L-SD), at 76,237 to 77,622 Rv0069c Position (kb) 75 76 77Strain fitness (log2 ratio) -3 -2 -1 0 1at 74.563 kb on - strandat 74.722 kb on + strand, within Rv0067cat 74.761 kb on + strand, within Rv0067cat 75.156 kb on + strandat 75.189 kb on + strandat 75.190 kb on - strandat 75.286 kb on + strandat 75.287 kb on - strandat 75.613 kb on + strand, within Rv0068at 75.613 kb on + strand, within Rv0068at 75.613 kb on + strand, within Rv0068at 75.614 kb on - strand, within Rv0068at 75.614 kb on - strand, within Rv0068at 75.614 kb on - strand, within Rv0068at 75.614 kb on - strand, within Rv0068at 75.614 kb on - strand, within Rv0068at 75.616 kb on - strand, within Rv0068at 76.070 kb on - strand, within Rv0068at 76.298 kb on + strandat 77.006 kb on - strand, within Rv0069c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Hydrogen peroxide 2.5 mM; pH 6.5
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74,563 - +0.3
74,722 + Rv0067c 0.16 -0.2
74,761 + Rv0067c 0.23 -3.1
75,156 + +0.4
75,189 + +0.8
75,190 - -0.6
75,286 + -3.2
75,287 - -1.3
75,613 + Rv0068 0.34 +1.5
75,613 + Rv0068 0.34 -0.2
75,613 + Rv0068 0.34 +1.6
75,614 - Rv0068 0.34 +0.3
75,614 - Rv0068 0.34 +0.2
75,614 - Rv0068 0.34 +0.4
75,614 - Rv0068 0.34 -0.3
75,614 - Rv0068 0.34 -0.7
75,616 - Rv0068 0.35 +0.8
76,070 - Rv0068 0.84 -0.2
76,298 + -0.2
77,006 - Rv0069c 0.55 +0.3

Or see this region's nucleotide sequence