Experiment: 7H9 with nitrite 1.5 mM; pH 5.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv3261 and Rv3262 overlap by 4 nucleotides Rv3262 and Rv3263 are separated by 295 nucleotides
Rv3261: Rv3261 - Probable F420 biosynthesis protein FbiA, at 3,640,543 to 3,641,538
Rv3261
Rv3262: Rv3262 - Probable F420 biosynthesis protein FbiB, at 3,641,535 to 3,642,881
Rv3262
Rv3263: Rv3263 - Probable DNA methylase (modification methylase) (methyltransferase), at 3,643,177 to 3,644,838
Rv3263
Position (kb)
3641
3642
3643 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3640.741 kb on + strand, within Rv3261 at 3640.742 kb on - strand, within Rv3261 at 3640.742 kb on - strand, within Rv3261 at 3640.742 kb on - strand, within Rv3261 at 3641.003 kb on + strand, within Rv3261 at 3641.887 kb on + strand, within Rv3262 at 3642.060 kb on + strand, within Rv3262 at 3642.061 kb on - strand, within Rv3262 at 3642.526 kb on + strand, within Rv3262 at 3643.037 kb on + strand at 3643.114 kb on + strand at 3643.114 kb on + strand at 3643.115 kb on - strand at 3643.219 kb on + strand at 3643.220 kb on - strand at 3643.240 kb on - strand at 3643.240 kb on - strand at 3643.240 kb on - strand at 3643.304 kb on - strand at 3643.306 kb on + strand at 3643.306 kb on + strand at 3643.306 kb on + strand at 3643.307 kb on - strand at 3643.507 kb on - strand, within Rv3263 at 3643.634 kb on + strand, within Rv3263 at 3643.764 kb on - strand, within Rv3263 at 3643.764 kb on - strand, within Rv3263 at 3643.807 kb on - strand, within Rv3263
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with nitrite 1.5 mM; pH 5.5 remove 3,640,741 + Rv3261 0.20 +0.1 3,640,742 - Rv3261 0.20 -1.3 3,640,742 - Rv3261 0.20 +1.3 3,640,742 - Rv3261 0.20 +0.2 3,641,003 + Rv3261 0.46 +0.4 3,641,887 + Rv3262 0.26 -0.4 3,642,060 + Rv3262 0.39 -0.4 3,642,061 - Rv3262 0.39 -1.2 3,642,526 + Rv3262 0.74 -2.7 3,643,037 + +0.4 3,643,114 + +0.1 3,643,114 + +2.1 3,643,115 - -0.3 3,643,219 + +0.1 3,643,220 - +2.5 3,643,240 - +0.4 3,643,240 - -0.8 3,643,240 - -0.3 3,643,304 - +1.0 3,643,306 + -0.1 3,643,306 + -0.4 3,643,306 + +1.3 3,643,307 - -0.7 3,643,507 - Rv3263 0.20 +0.3 3,643,634 + Rv3263 0.27 -0.5 3,643,764 - Rv3263 0.35 +0.3 3,643,764 - Rv3263 0.35 +0.7 3,643,807 - Rv3263 0.38 -1.1
Or see this region's nucleotide sequence