Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3216

Experiment: 7H9 with nitrite 1.5 mM; pH 5.5

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	7H9 with nitrite 1.5 mM; pH 5.5
3,593,377	+				-0.8
3,593,489	-		Rv3216	0.25	+0.7
3,593,613	+		Rv3216	0.50	-0.7
3,593,614	-		Rv3216	0.51	-0.4
3,593,614	-		Rv3216	0.51	-0.8
3,593,614	-		Rv3216	0.51	-1.1
3,593,614	-		Rv3216	0.51	-1.6
3,593,709	+		Rv3216	0.70	-1.4
3,593,763	+		Rv3216	0.81	-0.4
3,593,763	+		Rv3216	0.81	-0.9
3,593,801	+		Rv3216	0.89	-0.2
3,593,801	+		Rv3216	0.89	+0.2
3,593,802	-		Rv3216	0.89	-0.6
3,593,802	-		Rv3216	0.89	+0.7
3,593,805	+				-0.2
3,593,805	+				-0.9
3,593,898	-		Rv3217c	0.22	-0.9
3,593,967	+		Rv3217c	0.38	+1.7
3,593,968	-		Rv3217c	0.38	-0.4
3,594,246	+				-2.3
3,594,247	-				-0.3
3,594,343	-				-0.8
3,594,343	-				-0.6
3,594,454	+				-0.6
3,594,667	+		Rv3218	0.21	-1.8
3,594,667	+		Rv3218	0.21	+0.3
3,594,667	+		Rv3218	0.21	-0.7
3,594,763	+		Rv3218	0.31	-1.5
3,594,763	+		Rv3218	0.31	-0.3
3,594,764	-		Rv3218	0.31	+0.1
3,594,764	-		Rv3218	0.31	-0.3
3,594,764	-		Rv3218	0.31	-0.2
3,594,766	+		Rv3218	0.31	-0.8
3,594,766	+		Rv3218	0.31	+0.2
3,594,767	-		Rv3218	0.31	+1.7
3,594,767	-		Rv3218	0.31	-1.1