Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0121c

Experiment: 7H9 with nitrite 1.5 mM; pH 5.5

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0120c and Rv0121c are separated by 136 nucleotidesRv0121c and Rv0122 are separated by 148 nucleotidesRv0122 and Rv0123 overlap by 4 nucleotides Rv0120c: Rv0120c - Probable elongation factor G FusA2 (EF-G), at 145,627 to 147,771 Rv0120c Rv0121c: Rv0121c - Conserved protein, at 147,908 to 148,342 Rv0121c Rv0122: Rv0122 - Hypothetical protein, at 148,491 to 148,859 Rv0122 Rv0123: Rv0123 - Unknown protein, at 148,856 to 149,224 Rv0123 Position (kb) 147 148 149Strain fitness (log2 ratio) -1 0 1at 147.197 kb on + strand, within Rv0120cat 147.245 kb on + strand, within Rv0120cat 147.245 kb on + strand, within Rv0120cat 147.253 kb on + strand, within Rv0120cat 147.479 kb on + strand, within Rv0120cat 147.825 kb on + strandat 147.826 kb on - strandat 148.095 kb on + strand, within Rv0121cat 148.353 kb on + strandat 148.353 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with nitrite 1.5 mM; pH 5.5
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147,197 + Rv0120c 0.73 +0.1
147,245 + Rv0120c 0.75 +0.0
147,245 + Rv0120c 0.75 -0.3
147,253 + Rv0120c 0.76 +0.2
147,479 + Rv0120c 0.86 +0.8
147,825 + -0.7
147,826 - +0.2
148,095 + Rv0121c 0.43 -1.1
148,353 + +0.7
148,353 + +0.4

Or see this region's nucleotide sequence