Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0851c

Experiment: 7H9 with nitrite 1.5 mM; pH 5.5

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0849 and Rv0850 overlap by 4 nucleotidesRv0850 and Rv0851c overlap by 4 nucleotidesRv0851c and Rv0852 are separated by 90 nucleotidesRv0852 and Rv0853c are separated by 40 nucleotides Rv0849: Rv0849 - Probable conserved integral membrane transport protein, at 946,056 to 947,315 Rv0849 Rv0850: Rv0850 - Putative transposase (fragment), at 947,312 to 947,644 Rv0850 Rv0851c: Rv0851c - Probable short-chain type dehydrogenase/reductase, at 947,641 to 948,468 Rv0851c Rv0852: Rv0852 - Possible fatty-acid-CoA ligase FadD16 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase), at 948,559 to 949,395 Rv0852 Rv0853c: Rv0853c - Probable pyruvate or indole-3-pyruvate decarboxylase Pdc, at 949,436 to 951,118 Rv0853c Position (kb) 947 948 949Strain fitness (log2 ratio) -1 0 1 2at 946.725 kb on + strand, within Rv0849at 946.746 kb on + strand, within Rv0849at 946.794 kb on + strand, within Rv0849at 946.795 kb on - strand, within Rv0849at 947.109 kb on + strand, within Rv0849at 947.109 kb on + strand, within Rv0849at 947.392 kb on - strand, within Rv0850at 947.742 kb on - strand, within Rv0851cat 947.742 kb on - strand, within Rv0851cat 948.481 kb on + strandat 948.575 kb on - strandat 948.819 kb on + strand, within Rv0852at 948.935 kb on + strand, within Rv0852at 949.068 kb on + strand, within Rv0852at 949.068 kb on + strand, within Rv0852at 949.336 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with nitrite 1.5 mM; pH 5.5
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946,725 + Rv0849 0.53 -0.9
946,746 + Rv0849 0.55 -0.6
946,794 + Rv0849 0.59 +1.0
946,795 - Rv0849 0.59 -1.1
947,109 + Rv0849 0.84 -0.0
947,109 + Rv0849 0.84 +1.2
947,392 - Rv0850 0.24 -0.1
947,742 - Rv0851c 0.12 +2.1
947,742 - Rv0851c 0.12 -0.0
948,481 + -0.4
948,575 - -0.3
948,819 + Rv0852 0.31 -0.3
948,935 + Rv0852 0.45 -0.6
949,068 + Rv0852 0.61 -0.8
949,068 + Rv0852 0.61 -0.3
949,336 + -0.5

Or see this region's nucleotide sequence