Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0188

Experiment: 7H9 with nitrite 1.5 mM; pH 5.5

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0186A and Rv0187 are separated by 153 nucleotidesRv0187 and Rv0188 are separated by 118 nucleotidesRv0188 and Rv0189c are separated by 78 nucleotides Rv0186A: Rv0186A - Metallothionein, MymT, at 218,390 to 218,551 Rv0186A Rv0187: Rv0187 - Probable O-methyltransferase, at 218,705 to 219,367 Rv0187 Rv0188: Rv0188 - Probable conserved transmembrane protein, at 219,486 to 219,917 Rv0188 Rv0189c: Rv0189c - Probable dihydroxy-acid dehydratase IlvD (dad), at 219,996 to 221,723 Rv0189c Position (kb) 219 220Strain fitness (log2 ratio) -2 -1 0 1at 218.596 kb on + strandat 218.610 kb on + strandat 218.614 kb on + strandat 218.615 kb on - strandat 218.820 kb on + strand, within Rv0187at 218.981 kb on + strand, within Rv0187at 218.981 kb on + strand, within Rv0187at 218.982 kb on - strand, within Rv0187at 219.148 kb on + strand, within Rv0187at 219.205 kb on + strand, within Rv0187at 219.206 kb on - strand, within Rv0187at 219.298 kb on - strand, within Rv0187at 219.492 kb on - strandat 219.613 kb on - strand, within Rv0188at 219.860 kb on - strand, within Rv0188at 219.915 kb on + strandat 219.916 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with nitrite 1.5 mM; pH 5.5
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218,596 + +0.1
218,610 + -1.1
218,614 + -0.5
218,615 - -0.5
218,820 + Rv0187 0.17 -1.1
218,981 + Rv0187 0.42 -0.1
218,981 + Rv0187 0.42 +1.7
218,982 - Rv0187 0.42 -0.0
219,148 + Rv0187 0.67 -0.3
219,205 + Rv0187 0.75 +1.1
219,206 - Rv0187 0.76 -1.9
219,298 - Rv0187 0.89 -0.9
219,492 - -1.4
219,613 - Rv0188 0.29 -0.6
219,860 - Rv0188 0.87 -0.6
219,915 + -1.2
219,916 - +0.3

Or see this region's nucleotide sequence