Experiment: R2A with 1-octen-3-ol 1.3568235 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE51_RS08425 and ABIE51_RS08430 are separated by 57 nucleotides ABIE51_RS08430 and ABIE51_RS08435 are separated by 109 nucleotides ABIE51_RS08435 and ABIE51_RS08440 are separated by 66 nucleotides
ABIE51_RS08425: ABIE51_RS08425 - RNA polymerase sigma-70 factor, at 1,792,178 to 1,793,038
_RS08425
ABIE51_RS08430: ABIE51_RS08430 - carboxymuconolactone decarboxylase family protein, at 1,793,096 to 1,793,545
_RS08430
ABIE51_RS08435: ABIE51_RS08435 - hypothetical protein, at 1,793,655 to 1,794,071
_RS08435
ABIE51_RS08440: ABIE51_RS08440 - HAMP domain-containing sensor histidine kinase, at 1,794,138 to 1,795,409
_RS08440
Position (kb)
1793
1794
1795 Strain fitness (log2 ratio)
-1
0
1 at 1792.845 kb on - strand, within ABIE51_RS08425 at 1792.995 kb on + strand at 1792.996 kb on - strand at 1792.996 kb on - strand at 1793.521 kb on - strand at 1793.521 kb on - strand at 1793.521 kb on - strand at 1793.588 kb on + strand at 1793.589 kb on - strand at 1793.589 kb on - strand at 1793.993 kb on - strand, within ABIE51_RS08435 at 1794.103 kb on + strand at 1794.104 kb on - strand at 1794.104 kb on - strand at 1794.287 kb on - strand, within ABIE51_RS08440 at 1794.805 kb on + strand, within ABIE51_RS08440 at 1794.806 kb on - strand, within ABIE51_RS08440 at 1794.806 kb on - strand, within ABIE51_RS08440
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 1.3568235 mM remove 1,792,845 - ABIE51_RS08425 0.77 -0.5 1,792,995 + -0.6 1,792,996 - -1.4 1,792,996 - +1.5 1,793,521 - -0.3 1,793,521 - +0.3 1,793,521 - +0.1 1,793,588 + +0.8 1,793,589 - -0.1 1,793,589 - -0.3 1,793,993 - ABIE51_RS08435 0.81 -0.8 1,794,103 + +0.1 1,794,104 - -0.1 1,794,104 - -0.0 1,794,287 - ABIE51_RS08440 0.12 +0.0 1,794,805 + ABIE51_RS08440 0.52 -0.0 1,794,806 - ABIE51_RS08440 0.53 -0.1 1,794,806 - ABIE51_RS08440 0.53 -0.4
Or see this region's nucleotide sequence