Experiment: R2A with 1-octen-3-ol 1.3568235 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE51_RS08000 and ABIE51_RS08005 are separated by 94 nucleotides ABIE51_RS08005 and ABIE51_RS08010 are separated by 170 nucleotides ABIE51_RS08010 and ABIE51_RS08015 are separated by 115 nucleotides
ABIE51_RS08000: ABIE51_RS08000 - hypothetical protein, at 1,703,919 to 1,704,419
_RS08000
ABIE51_RS08005: ABIE51_RS08005 - ferredoxin FdxA, at 1,704,514 to 1,704,837
_RS08005
ABIE51_RS08010: ABIE51_RS08010 - tRNA-Glu, at 1,705,008 to 1,705,083
_RS08010
ABIE51_RS08015: ABIE51_RS08015 - polynucleotide adenylyltransferase PcnB, at 1,705,199 to 1,706,524
_RS08015
Position (kb)
1705
1706 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1704.262 kb on - strand, within ABIE51_RS08000 at 1704.273 kb on + strand, within ABIE51_RS08000 at 1704.274 kb on - strand, within ABIE51_RS08000 at 1704.274 kb on - strand, within ABIE51_RS08000 at 1704.324 kb on + strand, within ABIE51_RS08000 at 1704.324 kb on + strand, within ABIE51_RS08000 at 1704.325 kb on - strand, within ABIE51_RS08000 at 1704.417 kb on + strand at 1704.417 kb on + strand at 1704.417 kb on + strand at 1704.418 kb on - strand at 1704.904 kb on + strand at 1704.904 kb on + strand at 1704.904 kb on + strand at 1704.904 kb on + strand at 1704.904 kb on + strand at 1704.904 kb on + strand at 1704.905 kb on - strand at 1704.905 kb on - strand at 1704.905 kb on - strand at 1704.907 kb on + strand at 1704.908 kb on - strand at 1704.908 kb on - strand at 1704.908 kb on - strand at 1704.908 kb on - strand at 1704.908 kb on - strand at 1704.913 kb on - strand at 1705.011 kb on + strand at 1705.012 kb on - strand at 1705.012 kb on - strand at 1705.038 kb on + strand, within ABIE51_RS08010 at 1705.039 kb on - strand, within ABIE51_RS08010 at 1705.039 kb on - strand, within ABIE51_RS08010 at 1705.062 kb on + strand, within ABIE51_RS08010 at 1705.070 kb on + strand, within ABIE51_RS08010 at 1705.132 kb on + strand at 1705.132 kb on + strand at 1705.132 kb on + strand at 1705.132 kb on + strand at 1705.132 kb on + strand at 1705.132 kb on + strand at 1705.132 kb on + strand at 1705.132 kb on + strand at 1705.133 kb on - strand at 1705.133 kb on - strand at 1705.133 kb on - strand at 1705.133 kb on - strand at 1705.135 kb on + strand at 1705.135 kb on + strand at 1705.135 kb on + strand at 1705.135 kb on + strand at 1705.135 kb on + strand at 1705.136 kb on - strand at 1705.136 kb on - strand at 1705.136 kb on - strand at 1705.136 kb on - strand at 1705.136 kb on - strand at 1705.136 kb on - strand at 1705.136 kb on - strand at 1705.136 kb on - strand at 1705.136 kb on - strand at 1705.136 kb on - strand at 1705.136 kb on - strand at 1705.138 kb on - strand at 1705.138 kb on - strand at 1705.138 kb on - strand at 1705.138 kb on - strand at 1705.138 kb on - strand at 1705.276 kb on + strand at 1705.276 kb on + strand at 1705.276 kb on + strand at 1705.295 kb on + strand at 1705.325 kb on + strand at 1705.325 kb on + strand at 1705.326 kb on - strand at 1705.326 kb on - strand at 1705.476 kb on - strand, within ABIE51_RS08015 at 1705.580 kb on + strand, within ABIE51_RS08015 at 1705.580 kb on + strand, within ABIE51_RS08015 at 1705.580 kb on + strand, within ABIE51_RS08015 at 1705.581 kb on - strand, within ABIE51_RS08015 at 1705.581 kb on - strand, within ABIE51_RS08015 at 1705.581 kb on - strand, within ABIE51_RS08015 at 1705.581 kb on - strand, within ABIE51_RS08015 at 1705.634 kb on + strand, within ABIE51_RS08015 at 1705.634 kb on + strand, within ABIE51_RS08015 at 1705.637 kb on + strand, within ABIE51_RS08015 at 1705.637 kb on + strand, within ABIE51_RS08015 at 1705.638 kb on - strand, within ABIE51_RS08015 at 1705.668 kb on - strand, within ABIE51_RS08015
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 1.3568235 mM remove 1,704,262 - ABIE51_RS08000 0.68 +1.6 1,704,273 + ABIE51_RS08000 0.71 -0.4 1,704,274 - ABIE51_RS08000 0.71 +0.1 1,704,274 - ABIE51_RS08000 0.71 -0.0 1,704,324 + ABIE51_RS08000 0.81 +0.6 1,704,324 + ABIE51_RS08000 0.81 -1.6 1,704,325 - ABIE51_RS08000 0.81 +0.2 1,704,417 + +0.7 1,704,417 + +0.7 1,704,417 + +3.1 1,704,418 - +0.5 1,704,904 + +0.8 1,704,904 + -0.2 1,704,904 + -0.3 1,704,904 + +0.3 1,704,904 + -0.1 1,704,904 + -0.9 1,704,905 - +0.4 1,704,905 - -0.7 1,704,905 - -0.1 1,704,907 + -0.5 1,704,908 - -0.1 1,704,908 - -0.5 1,704,908 - -0.3 1,704,908 - +0.3 1,704,908 - -0.7 1,704,913 - -0.1 1,705,011 + -0.3 1,705,012 - -0.1 1,705,012 - -0.2 1,705,038 + ABIE51_RS08010 0.39 +0.3 1,705,039 - ABIE51_RS08010 0.41 -0.5 1,705,039 - ABIE51_RS08010 0.41 -0.0 1,705,062 + ABIE51_RS08010 0.71 -0.6 1,705,070 + ABIE51_RS08010 0.82 -0.8 1,705,132 + -0.5 1,705,132 + -0.2 1,705,132 + -1.0 1,705,132 + -0.2 1,705,132 + -1.8 1,705,132 + +0.6 1,705,132 + -1.1 1,705,132 + +0.1 1,705,133 - +0.4 1,705,133 - +0.3 1,705,133 - -0.8 1,705,133 - +1.1 1,705,135 + -1.1 1,705,135 + -1.0 1,705,135 + +0.1 1,705,135 + +0.4 1,705,135 + -0.8 1,705,136 - -0.3 1,705,136 - +0.5 1,705,136 - -0.7 1,705,136 - +0.3 1,705,136 - +0.5 1,705,136 - +0.9 1,705,136 - +0.2 1,705,136 - +0.5 1,705,136 - +0.3 1,705,136 - +0.5 1,705,136 - +0.4 1,705,138 - +1.1 1,705,138 - +0.8 1,705,138 - -0.0 1,705,138 - +0.3 1,705,138 - -0.7 1,705,276 + -0.5 1,705,276 + +0.6 1,705,276 + +0.1 1,705,295 + -0.9 1,705,325 + -0.1 1,705,325 + +0.3 1,705,326 - +0.0 1,705,326 - -0.1 1,705,476 - ABIE51_RS08015 0.21 +0.7 1,705,580 + ABIE51_RS08015 0.29 +1.1 1,705,580 + ABIE51_RS08015 0.29 +1.2 1,705,580 + ABIE51_RS08015 0.29 +0.4 1,705,581 - ABIE51_RS08015 0.29 +1.1 1,705,581 - ABIE51_RS08015 0.29 +0.2 1,705,581 - ABIE51_RS08015 0.29 +1.0 1,705,581 - ABIE51_RS08015 0.29 +0.4 1,705,634 + ABIE51_RS08015 0.33 +0.2 1,705,634 + ABIE51_RS08015 0.33 -1.0 1,705,637 + ABIE51_RS08015 0.33 -1.8 1,705,637 + ABIE51_RS08015 0.33 +0.1 1,705,638 - ABIE51_RS08015 0.33 +0.8 1,705,668 - ABIE51_RS08015 0.35 +0.1
Or see this region's nucleotide sequence