Experiment: R2A with 1-octen-3-ol 1.3568235 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE51_RS07275 and ABIE51_RS07280 overlap by 1 nucleotides ABIE51_RS07280 and ABIE51_RS07285 are separated by 126 nucleotides ABIE51_RS07285 and ABIE51_RS07290 are separated by 15 nucleotides ABIE51_RS07290 and ABIE51_RS07295 overlap by 13 nucleotides
ABIE51_RS07275: ABIE51_RS07275 - hypothetical protein, at 1,541,596 to 1,541,796
_RS07275
ABIE51_RS07280: ABIE51_RS07280 - hypothetical protein, at 1,541,796 to 1,542,074
_RS07280
ABIE51_RS07285: ABIE51_RS07285 - hypothetical protein, at 1,542,201 to 1,542,491
_RS07285
ABIE51_RS07290: ABIE51_RS07290 - DUF5681 domain-containing protein, at 1,542,507 to 1,542,758
_RS07290
ABIE51_RS07295: ABIE51_RS07295 - hypothetical protein, at 1,542,746 to 1,544,014
_RS07295
Position (kb)
1542
1543 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1541.521 kb on - strand at 1541.563 kb on - strand at 1541.563 kb on - strand at 1541.606 kb on + strand at 1541.688 kb on - strand, within ABIE51_RS07275 at 1541.791 kb on - strand at 1541.836 kb on - strand, within ABIE51_RS07280 at 1541.836 kb on - strand, within ABIE51_RS07280 at 1541.843 kb on + strand, within ABIE51_RS07280 at 1541.962 kb on + strand, within ABIE51_RS07280 at 1541.963 kb on - strand, within ABIE51_RS07280 at 1542.122 kb on + strand at 1542.123 kb on - strand at 1542.123 kb on - strand at 1542.123 kb on - strand at 1542.123 kb on - strand at 1542.123 kb on - strand at 1542.123 kb on - strand at 1542.190 kb on - strand at 1542.220 kb on + strand at 1542.220 kb on + strand at 1542.220 kb on + strand at 1542.220 kb on + strand at 1542.221 kb on - strand at 1542.221 kb on - strand at 1542.221 kb on - strand at 1542.221 kb on - strand at 1542.342 kb on + strand, within ABIE51_RS07285 at 1542.452 kb on - strand, within ABIE51_RS07285 at 1542.457 kb on + strand, within ABIE51_RS07285 at 1542.468 kb on + strand at 1542.469 kb on - strand at 1542.469 kb on - strand at 1542.503 kb on - strand at 1542.689 kb on + strand, within ABIE51_RS07290 at 1542.756 kb on + strand at 1542.756 kb on + strand at 1542.797 kb on + strand at 1542.898 kb on + strand, within ABIE51_RS07295 at 1542.898 kb on + strand, within ABIE51_RS07295 at 1542.936 kb on - strand, within ABIE51_RS07295 at 1542.938 kb on + strand, within ABIE51_RS07295 at 1542.938 kb on + strand, within ABIE51_RS07295 at 1542.938 kb on + strand, within ABIE51_RS07295 at 1542.938 kb on + strand, within ABIE51_RS07295 at 1542.968 kb on + strand, within ABIE51_RS07295 at 1542.969 kb on - strand, within ABIE51_RS07295 at 1542.969 kb on - strand, within ABIE51_RS07295 at 1542.969 kb on - strand, within ABIE51_RS07295 at 1542.980 kb on + strand, within ABIE51_RS07295 at 1542.980 kb on + strand, within ABIE51_RS07295 at 1543.098 kb on - strand, within ABIE51_RS07295 at 1543.142 kb on - strand, within ABIE51_RS07295 at 1543.220 kb on - strand, within ABIE51_RS07295 at 1543.285 kb on - strand, within ABIE51_RS07295 at 1543.285 kb on - strand, within ABIE51_RS07295 at 1543.409 kb on + strand, within ABIE51_RS07295 at 1543.409 kb on + strand, within ABIE51_RS07295 at 1543.409 kb on + strand, within ABIE51_RS07295 at 1543.410 kb on - strand, within ABIE51_RS07295 at 1543.410 kb on - strand, within ABIE51_RS07295 at 1543.410 kb on - strand, within ABIE51_RS07295 at 1543.410 kb on - strand, within ABIE51_RS07295 at 1543.486 kb on + strand, within ABIE51_RS07295 at 1543.568 kb on + strand, within ABIE51_RS07295 at 1543.571 kb on + strand, within ABIE51_RS07295 at 1543.571 kb on + strand, within ABIE51_RS07295 at 1543.572 kb on - strand, within ABIE51_RS07295 at 1543.602 kb on - strand, within ABIE51_RS07295 at 1543.602 kb on - strand, within ABIE51_RS07295 at 1543.634 kb on + strand, within ABIE51_RS07295 at 1543.722 kb on - strand, within ABIE51_RS07295
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 1.3568235 mM remove 1,541,521 - -0.3 1,541,563 - +0.2 1,541,563 - +0.5 1,541,606 + -0.6 1,541,688 - ABIE51_RS07275 0.46 +0.3 1,541,791 - -0.6 1,541,836 - ABIE51_RS07280 0.14 +1.1 1,541,836 - ABIE51_RS07280 0.14 +0.9 1,541,843 + ABIE51_RS07280 0.17 -3.0 1,541,962 + ABIE51_RS07280 0.59 -0.5 1,541,963 - ABIE51_RS07280 0.60 +1.3 1,542,122 + +0.5 1,542,123 - -0.9 1,542,123 - -0.4 1,542,123 - -0.2 1,542,123 - +0.1 1,542,123 - -0.2 1,542,123 - +0.5 1,542,190 - -0.5 1,542,220 + +0.4 1,542,220 + +0.3 1,542,220 + +1.4 1,542,220 + -0.3 1,542,221 - +0.2 1,542,221 - +0.2 1,542,221 - +0.1 1,542,221 - +0.3 1,542,342 + ABIE51_RS07285 0.48 -1.5 1,542,452 - ABIE51_RS07285 0.86 -1.2 1,542,457 + ABIE51_RS07285 0.88 -0.4 1,542,468 + -0.3 1,542,469 - -1.0 1,542,469 - +1.0 1,542,503 - +0.1 1,542,689 + ABIE51_RS07290 0.72 -0.6 1,542,756 + +0.2 1,542,756 + +0.4 1,542,797 + -0.3 1,542,898 + ABIE51_RS07295 0.12 +0.8 1,542,898 + ABIE51_RS07295 0.12 -0.1 1,542,936 - ABIE51_RS07295 0.15 -0.0 1,542,938 + ABIE51_RS07295 0.15 -0.2 1,542,938 + ABIE51_RS07295 0.15 +0.0 1,542,938 + ABIE51_RS07295 0.15 -0.5 1,542,938 + ABIE51_RS07295 0.15 +0.1 1,542,968 + ABIE51_RS07295 0.17 -0.2 1,542,969 - ABIE51_RS07295 0.18 +0.0 1,542,969 - ABIE51_RS07295 0.18 +0.0 1,542,969 - ABIE51_RS07295 0.18 +1.1 1,542,980 + ABIE51_RS07295 0.18 -0.5 1,542,980 + ABIE51_RS07295 0.18 +0.3 1,543,098 - ABIE51_RS07295 0.28 +0.4 1,543,142 - ABIE51_RS07295 0.31 +0.5 1,543,220 - ABIE51_RS07295 0.37 -0.6 1,543,285 - ABIE51_RS07295 0.42 -3.0 1,543,285 - ABIE51_RS07295 0.42 -0.5 1,543,409 + ABIE51_RS07295 0.52 -0.5 1,543,409 + ABIE51_RS07295 0.52 -0.4 1,543,409 + ABIE51_RS07295 0.52 -1.2 1,543,410 - ABIE51_RS07295 0.52 -0.0 1,543,410 - ABIE51_RS07295 0.52 +0.8 1,543,410 - ABIE51_RS07295 0.52 -0.3 1,543,410 - ABIE51_RS07295 0.52 -0.0 1,543,486 + ABIE51_RS07295 0.58 -0.2 1,543,568 + ABIE51_RS07295 0.65 +0.1 1,543,571 + ABIE51_RS07295 0.65 -1.2 1,543,571 + ABIE51_RS07295 0.65 -0.5 1,543,572 - ABIE51_RS07295 0.65 -0.3 1,543,602 - ABIE51_RS07295 0.67 -1.0 1,543,602 - ABIE51_RS07295 0.67 -0.5 1,543,634 + ABIE51_RS07295 0.70 +0.5 1,543,722 - ABIE51_RS07295 0.77 -0.1
Or see this region's nucleotide sequence