Experiment: pH 4.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0174 and Rv0175 are separated by 35 nucleotides Rv0175 and Rv0176 overlap by 4 nucleotides Rv0176 and Rv0177 overlap by 4 nucleotides Rv0177 and Rv0178 overlap by 34 nucleotides
Rv0174: Rv0174 - Mce-family protein Mce1F, at 205,231 to 206,778
Rv0174
Rv0175: Rv0175 - Probable conserved Mce associated membrane protein, at 206,814 to 207,455
Rv0175
Rv0176: Rv0176 - Probable conserved Mce associated transmembrane protein, at 207,452 to 208,420
Rv0176
Rv0177: Rv0177 - Probable conserved Mce associated protein, at 208,417 to 208,971
Rv0177
Rv0178: Rv0178 - Probable conserved Mce associated membrane protein, at 208,938 to 209,672
Rv0178
Position (kb)
207
208
209 Strain fitness (log2 ratio)
-2
-1
0
1 at 206.623 kb on - strand, within Rv0174 at 206.623 kb on - strand, within Rv0174 at 206.636 kb on - strand at 206.711 kb on - strand at 206.711 kb on - strand at 206.795 kb on + strand at 206.808 kb on - strand at 207.420 kb on + strand at 207.422 kb on + strand at 207.423 kb on - strand at 207.423 kb on - strand at 208.010 kb on + strand, within Rv0176 at 208.010 kb on + strand, within Rv0176 at 208.092 kb on - strand, within Rv0176 at 208.152 kb on - strand, within Rv0176 at 208.208 kb on - strand, within Rv0176 at 208.295 kb on - strand, within Rv0176 at 208.295 kb on - strand, within Rv0176 at 208.314 kb on - strand, within Rv0176 at 208.315 kb on + strand, within Rv0176 at 208.316 kb on - strand, within Rv0176 at 208.726 kb on + strand, within Rv0177 at 208.827 kb on + strand, within Rv0177 at 208.828 kb on - strand, within Rv0177 at 209.247 kb on + strand, within Rv0178
Per-strain Table
Position Strand Gene LocusTag Fraction pH 4.5 remove 206,623 - Rv0174 0.90 -1.7 206,623 - Rv0174 0.90 +0.1 206,636 - +1.7 206,711 - -0.6 206,711 - +0.2 206,795 + -0.2 206,808 - -1.5 207,420 + -1.1 207,422 + -0.9 207,423 - -1.6 207,423 - -0.6 208,010 + Rv0176 0.58 -0.6 208,010 + Rv0176 0.58 -1.2 208,092 - Rv0176 0.66 -1.7 208,152 - Rv0176 0.72 -1.6 208,208 - Rv0176 0.78 -1.3 208,295 - Rv0176 0.87 +0.0 208,295 - Rv0176 0.87 -1.5 208,314 - Rv0176 0.89 -1.7 208,315 + Rv0176 0.89 -1.9 208,316 - Rv0176 0.89 -1.3 208,726 + Rv0177 0.56 -0.1 208,827 + Rv0177 0.74 -2.0 208,828 - Rv0177 0.74 +0.2 209,247 + Rv0178 0.42 -0.7
Or see this region's nucleotide sequence