Experiment: 7H9 with hypochlorite 0.4 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv2270 and Rv2271 are separated by 106 nucleotides Rv2271 and Rv2272 are separated by 105 nucleotides Rv2272 and Rv2273 overlap by 4 nucleotides Rv2273 and Rv2274c are separated by 56 nucleotides Rv2274c and Rv2274A are separated by 33 nucleotides Rv2274A and Rv2275 overlap by 205 nucleotides
Rv2270: Rv2270 - Probable lipoprotein LppN, at 2,544,698 to 2,545,225
Rv2270
Rv2271: Rv2271 - Conserved hypothetical protein, at 2,545,332 to 2,545,631
Rv2271
Rv2272: Rv2272 - Probable conserved transmembrane protein, at 2,545,737 to 2,546,105
Rv2272
Rv2273: Rv2273 - Probable conserved transmembrane protein, at 2,546,102 to 2,546,431
Rv2273
Rv2274c: Rv2274c - Possible toxin MazF8, at 2,546,488 to 2,546,805
Rv2274c
Rv2274A: Rv2274A - Possible antitoxin MazE8, at 2,546,839 to 2,547,087
Rv2274A
Rv2275: Rv2275 - Conserved hypothetical protein, at 2,546,883 to 2,547,752
Rv2275
Position (kb)
2546
2547 Strain fitness (log2 ratio)
-1
0
1
2 at 2545.113 kb on - strand, within Rv2270 at 2545.136 kb on - strand, within Rv2270 at 2545.308 kb on + strand at 2545.455 kb on + strand, within Rv2271 at 2545.578 kb on + strand, within Rv2271 at 2545.597 kb on + strand, within Rv2271 at 2545.598 kb on - strand, within Rv2271 at 2545.654 kb on + strand at 2545.655 kb on - strand at 2545.655 kb on - strand at 2545.682 kb on + strand at 2546.032 kb on - strand, within Rv2272 at 2546.291 kb on + strand, within Rv2273 at 2546.291 kb on + strand, within Rv2273 at 2546.291 kb on + strand, within Rv2273 at 2546.305 kb on - strand, within Rv2273 at 2546.490 kb on - strand at 2546.707 kb on + strand, within Rv2274c at 2546.708 kb on - strand, within Rv2274c at 2546.779 kb on - strand at 2546.800 kb on - strand at 2547.160 kb on - strand, within Rv2275
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with hypochlorite 0.4 vol% remove 2,545,113 - Rv2270 0.79 -0.7 2,545,136 - Rv2270 0.83 +0.3 2,545,308 + -0.7 2,545,455 + Rv2271 0.41 -1.4 2,545,578 + Rv2271 0.82 +0.5 2,545,597 + Rv2271 0.88 -0.2 2,545,598 - Rv2271 0.89 -0.3 2,545,654 + -0.0 2,545,655 - +0.2 2,545,655 - -0.6 2,545,682 + +0.3 2,546,032 - Rv2272 0.80 +0.8 2,546,291 + Rv2273 0.57 -0.2 2,546,291 + Rv2273 0.57 -0.5 2,546,291 + Rv2273 0.57 -1.2 2,546,305 - Rv2273 0.62 -1.2 2,546,490 - -0.8 2,546,707 + Rv2274c 0.69 +0.1 2,546,708 - Rv2274c 0.69 +1.8 2,546,779 - +0.1 2,546,800 - +1.8 2,547,160 - Rv2275 0.32 +0.0
Or see this region's nucleotide sequence