Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3704c

Experiment: 7H9 with hypochlorite 0.4 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3703c and Rv3704c overlap by 4 nucleotidesRv3704c and Rv3705c are separated by 131 nucleotidesRv3705c and Rv3705A are separated by 128 nucleotides Rv3703c: Rv3703c - Conserved hypothetical protein, at 4,145,614 to 4,146,891 Rv3703c Rv3704c: Rv3704c - Glutamate--cysteine ligase GshA (gamma-glutamylcysteine synthetase) (gamma-ECS) (GCS) (gamma-glutamyl-L-cysteine synthetase), at 4,146,888 to 4,148,186 Rv3704c Rv3705c: Rv3705c - Conserved protein, at 4,148,318 to 4,148,962 Rv3705c Rv3705A: Rv3705A - Conserved hypothetical proline rich protein, at 4,149,091 to 4,149,480 Rv3705A Position (kb) 4146 4147 4148 4149Strain fitness (log2 ratio) -1 0 1 2at 4145.927 kb on + strand, within Rv3703cat 4145.927 kb on + strand, within Rv3703cat 4145.928 kb on - strand, within Rv3703cat 4145.928 kb on - strand, within Rv3703cat 4146.069 kb on - strand, within Rv3703cat 4146.225 kb on - strand, within Rv3703cat 4146.225 kb on - strand, within Rv3703cat 4146.463 kb on + strand, within Rv3703cat 4146.464 kb on - strand, within Rv3703cat 4146.596 kb on + strand, within Rv3703cat 4146.668 kb on + strand, within Rv3703cat 4146.669 kb on - strand, within Rv3703cat 4146.669 kb on - strand, within Rv3703cat 4147.366 kb on + strand, within Rv3704cat 4147.435 kb on + strand, within Rv3704cat 4147.435 kb on + strand, within Rv3704cat 4147.436 kb on - strand, within Rv3704cat 4147.436 kb on - strand, within Rv3704cat 4147.439 kb on + strand, within Rv3704cat 4147.440 kb on - strand, within Rv3704cat 4147.440 kb on - strand, within Rv3704cat 4147.440 kb on - strand, within Rv3704cat 4148.092 kb on + strandat 4148.093 kb on - strandat 4148.093 kb on - strandat 4148.186 kb on + strandat 4148.186 kb on + strandat 4148.186 kb on + strandat 4148.186 kb on + strandat 4148.405 kb on + strand, within Rv3705cat 4148.625 kb on - strand, within Rv3705cat 4148.715 kb on + strand, within Rv3705cat 4148.715 kb on + strand, within Rv3705cat 4148.716 kb on - strand, within Rv3705cat 4148.716 kb on - strand, within Rv3705cat 4148.760 kb on - strand, within Rv3705cat 4148.859 kb on + strand, within Rv3705cat 4148.918 kb on + strandat 4148.918 kb on + strandat 4148.919 kb on - strandat 4148.927 kb on + strandat 4148.968 kb on + strandat 4148.969 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with hypochlorite 0.4 vol%
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4,145,927 + Rv3703c 0.24 -0.8
4,145,927 + Rv3703c 0.24 -0.4
4,145,928 - Rv3703c 0.25 -0.3
4,145,928 - Rv3703c 0.25 -0.7
4,146,069 - Rv3703c 0.36 -1.0
4,146,225 - Rv3703c 0.48 -1.2
4,146,225 - Rv3703c 0.48 -0.7
4,146,463 + Rv3703c 0.66 -0.3
4,146,464 - Rv3703c 0.67 -0.8
4,146,596 + Rv3703c 0.77 -0.2
4,146,668 + Rv3703c 0.82 -0.6
4,146,669 - Rv3703c 0.83 -0.2
4,146,669 - Rv3703c 0.83 -1.3
4,147,366 + Rv3704c 0.37 -1.0
4,147,435 + Rv3704c 0.42 -1.1
4,147,435 + Rv3704c 0.42 -0.6
4,147,436 - Rv3704c 0.42 -1.2
4,147,436 - Rv3704c 0.42 -1.1
4,147,439 + Rv3704c 0.42 -0.5
4,147,440 - Rv3704c 0.42 +0.1
4,147,440 - Rv3704c 0.42 -0.9
4,147,440 - Rv3704c 0.42 -0.5
4,148,092 + -1.1
4,148,093 - -0.9
4,148,093 - +1.1
4,148,186 + -0.5
4,148,186 + -0.8
4,148,186 + -1.0
4,148,186 + -0.9
4,148,405 + Rv3705c 0.13 -0.2
4,148,625 - Rv3705c 0.48 +0.2
4,148,715 + Rv3705c 0.62 -0.6
4,148,715 + Rv3705c 0.62 +1.4
4,148,716 - Rv3705c 0.62 +0.3
4,148,716 - Rv3705c 0.62 -0.5
4,148,760 - Rv3705c 0.69 -0.5
4,148,859 + Rv3705c 0.84 -0.9
4,148,918 + +1.2
4,148,918 + -1.1
4,148,919 - +0.4
4,148,927 + -0.5
4,148,968 + +1.8
4,148,969 - -0.0

Or see this region's nucleotide sequence