Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3288c

Experiment: 7H9 with hypochlorite 0.4 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3286c and Rv3287c overlap by 4 nucleotidesRv3287c and Rv3288c are separated by 197 nucleotidesRv3288c and Rv3289c are separated by 34 nucleotidesRv3289c and Rv3290c are separated by 33 nucleotides Rv3286c: Rv3286c - Alternative RNA polymerase sigma factor SigF, at 3,668,169 to 3,668,954 Rv3286c Rv3287c: Rv3287c - Anti-sigma factor RsbW (sigma negative effector), at 3,668,951 to 3,669,388 Rv3287c Rv3288c: Rv3288c - Putative protein UsfY, at 3,669,586 to 3,669,999 Rv3288c Rv3289c: Rv3289c - Possible transmembrane protein, at 3,670,034 to 3,670,411 Rv3289c Rv3290c: Rv3290c - Probable L-lysine-epsilon aminotransferase Lat (L-lysine aminotransferase) (lysine 6-aminotransferase), at 3,670,445 to 3,671,794 Rv3290c Position (kb) 3669 3670Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3668.663 kb on + strand, within Rv3286cat 3668.916 kb on - strandat 3669.076 kb on + strand, within Rv3287cat 3669.077 kb on - strand, within Rv3287cat 3669.419 kb on - strandat 3669.473 kb on + strandat 3669.485 kb on - strandat 3669.515 kb on + strandat 3669.578 kb on + strandat 3669.578 kb on + strandat 3669.578 kb on + strandat 3669.578 kb on + strandat 3669.579 kb on - strandat 3669.579 kb on - strandat 3669.588 kb on - strandat 3669.624 kb on - strandat 3669.630 kb on - strand, within Rv3288cat 3669.804 kb on - strand, within Rv3288cat 3669.884 kb on + strand, within Rv3288cat 3669.884 kb on + strand, within Rv3288cat 3669.884 kb on + strand, within Rv3288cat 3669.884 kb on + strand, within Rv3288cat 3669.885 kb on - strand, within Rv3288cat 3669.885 kb on - strand, within Rv3288cat 3669.885 kb on - strand, within Rv3288cat 3669.885 kb on - strand, within Rv3288cat 3669.885 kb on - strand, within Rv3288cat 3669.918 kb on + strand, within Rv3288cat 3669.939 kb on + strand, within Rv3288cat 3669.962 kb on - strandat 3670.001 kb on + strandat 3670.001 kb on + strandat 3670.074 kb on - strand, within Rv3289cat 3670.074 kb on - strand, within Rv3289cat 3670.138 kb on + strand, within Rv3289cat 3670.138 kb on + strand, within Rv3289cat 3670.139 kb on - strand, within Rv3289cat 3670.139 kb on - strand, within Rv3289cat 3670.294 kb on + strand, within Rv3289cat 3670.401 kb on - strandat 3670.401 kb on - strandat 3670.446 kb on + strandat 3670.447 kb on - strandat 3670.621 kb on + strand, within Rv3290cat 3670.707 kb on + strand, within Rv3290cat 3670.708 kb on - strand, within Rv3290cat 3670.708 kb on - strand, within Rv3290cat 3670.711 kb on + strand, within Rv3290cat 3670.711 kb on + strand, within Rv3290cat 3670.712 kb on - strand, within Rv3290cat 3670.712 kb on - strand, within Rv3290cat 3670.790 kb on - strand, within Rv3290cat 3670.883 kb on + strand, within Rv3290cat 3670.883 kb on + strand, within Rv3290cat 3670.939 kb on - strand, within Rv3290cat 3670.976 kb on + strand, within Rv3290cat 3670.977 kb on - strand, within Rv3290c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with hypochlorite 0.4 vol%
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3,668,663 + Rv3286c 0.63 +1.8
3,668,916 - -0.3
3,669,076 + Rv3287c 0.29 +2.8
3,669,077 - Rv3287c 0.29 -0.2
3,669,419 - -0.3
3,669,473 + -0.1
3,669,485 - -0.6
3,669,515 + -0.3
3,669,578 + +0.6
3,669,578 + +0.0
3,669,578 + -1.3
3,669,578 + +0.2
3,669,579 - -2.1
3,669,579 - -1.6
3,669,588 - +0.2
3,669,624 - -0.1
3,669,630 - Rv3288c 0.11 -3.0
3,669,804 - Rv3288c 0.53 -0.7
3,669,884 + Rv3288c 0.72 +0.6
3,669,884 + Rv3288c 0.72 +1.8
3,669,884 + Rv3288c 0.72 +1.8
3,669,884 + Rv3288c 0.72 -0.1
3,669,885 - Rv3288c 0.72 -1.2
3,669,885 - Rv3288c 0.72 +0.4
3,669,885 - Rv3288c 0.72 -1.5
3,669,885 - Rv3288c 0.72 -0.6
3,669,885 - Rv3288c 0.72 -2.9
3,669,918 + Rv3288c 0.80 -0.5
3,669,939 + Rv3288c 0.85 -0.7
3,669,962 - -1.3
3,670,001 + +0.2
3,670,001 + -0.5
3,670,074 - Rv3289c 0.11 -1.7
3,670,074 - Rv3289c 0.11 -0.5
3,670,138 + Rv3289c 0.28 -0.4
3,670,138 + Rv3289c 0.28 -0.5
3,670,139 - Rv3289c 0.28 -0.2
3,670,139 - Rv3289c 0.28 -0.5
3,670,294 + Rv3289c 0.69 -2.6
3,670,401 - -1.0
3,670,401 - -0.2
3,670,446 + -0.8
3,670,447 - -0.4
3,670,621 + Rv3290c 0.13 +1.5
3,670,707 + Rv3290c 0.19 -0.2
3,670,708 - Rv3290c 0.19 -0.5
3,670,708 - Rv3290c 0.19 +1.1
3,670,711 + Rv3290c 0.20 -1.9
3,670,711 + Rv3290c 0.20 -1.9
3,670,712 - Rv3290c 0.20 -1.1
3,670,712 - Rv3290c 0.20 -0.1
3,670,790 - Rv3290c 0.26 -0.1
3,670,883 + Rv3290c 0.32 -0.4
3,670,883 + Rv3290c 0.32 -0.5
3,670,939 - Rv3290c 0.37 +0.3
3,670,976 + Rv3290c 0.39 -0.4
3,670,977 - Rv3290c 0.39 +1.6

Or see this region's nucleotide sequence