Experiment: 7H9 with hypochlorite 0.4 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv3220c and Rv3221c are separated by 16 nucleotides Rv3221c and Rv3221A are separated by 284 nucleotides Rv3221A and Rv3222c overlap by 4 nucleotides Rv3222c and Rv3223c overlap by 4 nucleotides Rv3223c and Rv3224 are separated by 299 nucleotides
Rv3220c: Rv3220c - Probable two component sensor kinase, at 3,596,029 to 3,597,534
Rv3220c
Rv3221c: Rv3221c - Biotinylated protein TB7.3, at 3,597,551 to 3,597,766
Rv3221c
Rv3221A: Rv3221A - Anti-sigma factor RshA, at 3,598,051 to 3,598,356
Rv3221A
Rv3222c: Rv3222c - Conserved hypothetical protein, at 3,598,353 to 3,598,904
Rv3222c
Rv3223c: Rv3223c - Alternative RNA polymerase sigma-E factor (sigma-24) SigH (RPOE), at 3,598,901 to 3,599,551
Rv3223c
Rv3224: Rv3224 - Possible iron-regulated short-chain dehydrogenase/reductase, at 3,599,851 to 3,600,699
Rv3224
Position (kb)
3598
3599 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3597.416 kb on + strand at 3597.552 kb on + strand at 3597.838 kb on - strand at 3597.838 kb on - strand at 3597.838 kb on - strand at 3597.847 kb on + strand at 3597.847 kb on + strand at 3597.848 kb on - strand at 3597.848 kb on - strand at 3597.848 kb on - strand at 3597.877 kb on + strand at 3597.985 kb on - strand at 3598.003 kb on + strand at 3598.003 kb on + strand at 3598.003 kb on + strand at 3598.039 kb on + strand at 3598.789 kb on - strand, within Rv3222c at 3598.789 kb on - strand, within Rv3222c at 3599.082 kb on + strand, within Rv3223c at 3599.083 kb on - strand, within Rv3223c at 3599.153 kb on + strand, within Rv3223c at 3599.271 kb on + strand, within Rv3223c at 3599.283 kb on + strand, within Rv3223c at 3599.284 kb on - strand, within Rv3223c at 3599.284 kb on - strand, within Rv3223c at 3599.356 kb on - strand, within Rv3223c at 3599.356 kb on - strand, within Rv3223c at 3599.431 kb on - strand, within Rv3223c at 3599.431 kb on - strand, within Rv3223c at 3599.559 kb on - strand at 3599.559 kb on - strand at 3599.690 kb on + strand at 3599.690 kb on + strand at 3599.691 kb on - strand at 3599.691 kb on - strand at 3599.691 kb on - strand at 3599.778 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with hypochlorite 0.4 vol% remove 3,597,416 + -1.0 3,597,552 + +1.8 3,597,838 - -1.3 3,597,838 - +1.8 3,597,838 - +0.0 3,597,847 + -1.8 3,597,847 + -1.9 3,597,848 - +1.8 3,597,848 - -0.4 3,597,848 - -0.2 3,597,877 + -1.1 3,597,985 - -0.4 3,598,003 + -0.1 3,598,003 + +1.0 3,598,003 + -0.7 3,598,039 + -0.5 3,598,789 - Rv3222c 0.79 -1.5 3,598,789 - Rv3222c 0.79 -3.3 3,599,082 + Rv3223c 0.28 -1.8 3,599,083 - Rv3223c 0.28 +1.8 3,599,153 + Rv3223c 0.39 -1.8 3,599,271 + Rv3223c 0.57 +0.4 3,599,283 + Rv3223c 0.59 +0.6 3,599,284 - Rv3223c 0.59 -0.5 3,599,284 - Rv3223c 0.59 +1.1 3,599,356 - Rv3223c 0.70 +1.0 3,599,356 - Rv3223c 0.70 -2.8 3,599,431 - Rv3223c 0.81 +1.8 3,599,431 - Rv3223c 0.81 +0.7 3,599,559 - -0.5 3,599,559 - +0.6 3,599,690 + -1.1 3,599,690 + -0.2 3,599,691 - -3.4 3,599,691 - -0.5 3,599,691 - -1.0 3,599,778 + -1.4
Or see this region's nucleotide sequence