Experiment: 7H9 with hypochlorite 0.4 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv2001 and Rv2002 are separated by 75 nucleotides Rv2002 and Rv2003c are separated by 120 nucleotides Rv2003c and Rv2004c are separated by 57 nucleotides
Rv2001: Rv2001 - Conserved hypothetical protein, at 2,246,832 to 2,247,584
Rv2001
Rv2002: Rv2002 - Possible 20-beta-hydroxysteroid dehydrogenase FabG3 (cortisone reductase) ((R)-20-hydroxysteroid dehydrogenase), at 2,247,660 to 2,248,442
Rv2002
Rv2003c: Rv2003c - Conserved hypothetical protein, at 2,248,563 to 2,249,420
Rv2003c
Rv2004c: Rv2004c - Conserved protein, at 2,249,478 to 2,250,974
Rv2004c
Position (kb)
2248
2249
2250 Strain fitness (log2 ratio)
-1
0
1 at 2247.580 kb on + strand at 2247.580 kb on + strand at 2247.713 kb on + strand at 2248.376 kb on - strand at 2248.440 kb on + strand at 2248.565 kb on - strand at 2248.593 kb on - strand at 2248.771 kb on + strand, within Rv2003c at 2248.823 kb on - strand, within Rv2003c at 2249.326 kb on - strand, within Rv2003c at 2249.504 kb on - strand at 2249.880 kb on + strand, within Rv2004c at 2250.140 kb on - strand, within Rv2004c
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with hypochlorite 0.4 vol% remove 2,247,580 + -1.0 2,247,580 + -0.2 2,247,713 + -0.5 2,248,376 - -0.2 2,248,440 + +1.1 2,248,565 - +0.1 2,248,593 - -0.3 2,248,771 + Rv2003c 0.24 -1.3 2,248,823 - Rv2003c 0.30 -0.1 2,249,326 - Rv2003c 0.89 -0.8 2,249,504 - -0.9 2,249,880 + Rv2004c 0.27 +0.2 2,250,140 - Rv2004c 0.44 -0.7
Or see this region's nucleotide sequence