Experiment: 7H9 with hypochlorite 0.4 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0585c and Rv0586 are separated by 137 nucleotides Rv0586 and Rv0587 overlap by 4 nucleotides Rv0587 and Rv0588 are separated by 1 nucleotides Rv0588 and Rv0589 are separated by 5 nucleotides
Rv0585c: Rv0585c - Probable conserved integral membrane protein, at 681,885 to 684,272
Rv0585c
Rv0586: Rv0586 - Probable transcriptional regulatory protein Mce2R (GntR-family), at 684,410 to 685,132
Rv0586
Rv0587: Rv0587 - Conserved hypothetical integral membrane protein YrbE2A, at 685,129 to 685,926
Rv0587
Rv0588: Rv0588 - Conserved hypothetical integral membrane protein YrbE2B, at 685,928 to 686,815
Rv0588
Rv0589: Rv0589 - Mce-family protein Mce2A, at 686,821 to 688,035
Rv0589
Position (kb) 685
686 Strain fitness (log2 ratio) -1
0
1 at 684.278 kb on - strand at 684.278 kb on - strand at 684.339 kb on + strand at 684.700 kb on + strand, within Rv0586 at 685.157 kb on - strand at 685.217 kb on + strand, within Rv0587 at 685.218 kb on - strand, within Rv0587 at 685.359 kb on + strand, within Rv0587 at 686.544 kb on - strand, within Rv0588 at 686.573 kb on + strand, within Rv0588 at 686.663 kb on + strand, within Rv0588 at 686.667 kb on - strand, within Rv0588 at 686.673 kb on - strand, within Rv0588 at 686.781 kb on - strand at 686.863 kb on + strand at 686.864 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with hypochlorite 0.4 vol% remove 684,278 - +0.8 684,278 - -0.7 684,339 + -0.7 684,700 + Rv0586 0.40 +0.0 685,157 - +0.5 685,217 + Rv0587 0.11 -0.7 685,218 - Rv0587 0.11 +0.5 685,359 + Rv0587 0.29 +1.7 686,544 - Rv0588 0.69 +1.0 686,573 + Rv0588 0.73 -0.1 686,663 + Rv0588 0.83 +0.1 686,667 - Rv0588 0.83 -0.2 686,673 - Rv0588 0.84 +0.5 686,781 - -0.2 686,863 + -0.7 686,864 - -0.6
Or see this region's nucleotide sequence