Experiment: 7H9 with hypochlorite 0.4 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0104 and Rv0105c are separated by 148 nucleotides Rv0105c and Rv0106 are separated by 109 nucleotides Rv0106 and Rv0107c are separated by 72 nucleotides
Rv0104: Rv0104 - Conserved hypothetical protein, at 122,317 to 123,831
Rv0104
Rv0105c: Rv0105c - 50S ribosomal protein L28-1 RpmB1, at 123,980 to 124,264
Rv0105c
Rv0106: Rv0106 - Conserved hypothetical protein, at 124,374 to 125,570
Rv0106
Rv0107c: Rv0107c - Probable cation-transporter ATPase I CtpI, at 125,643 to 130,541
Rv0107c
Position (kb)
124
125
126 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 123.521 kb on - strand, within Rv0104 at 123.522 kb on + strand, within Rv0104 at 123.612 kb on + strand, within Rv0104 at 123.830 kb on - strand at 123.896 kb on + strand at 123.896 kb on + strand at 123.906 kb on + strand at 123.906 kb on + strand at 123.910 kb on + strand at 123.910 kb on + strand at 123.910 kb on + strand at 123.911 kb on - strand at 123.911 kb on - strand at 123.911 kb on - strand at 123.911 kb on - strand at 123.911 kb on - strand at 124.379 kb on + strand at 124.390 kb on + strand at 124.391 kb on - strand at 125.015 kb on + strand, within Rv0106 at 125.293 kb on - strand, within Rv0106 at 125.293 kb on - strand, within Rv0106 at 125.293 kb on - strand, within Rv0106 at 125.377 kb on + strand, within Rv0106 at 125.470 kb on - strand at 125.605 kb on + strand at 125.606 kb on - strand at 125.620 kb on + strand at 125.644 kb on + strand at 125.666 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with hypochlorite 0.4 vol% remove 123,521 - Rv0104 0.79 -0.4 123,522 + Rv0104 0.80 -0.7 123,612 + Rv0104 0.85 -1.2 123,830 - +0.3 123,896 + +1.9 123,896 + +0.5 123,906 + +0.2 123,906 + -1.1 123,910 + -0.3 123,910 + -0.7 123,910 + -1.1 123,911 - -0.2 123,911 - -0.4 123,911 - -0.1 123,911 - -0.3 123,911 - -0.7 124,379 + -0.6 124,390 + +0.1 124,391 - +1.9 125,015 + Rv0106 0.54 -0.9 125,293 - Rv0106 0.77 -2.2 125,293 - Rv0106 0.77 +0.3 125,293 - Rv0106 0.77 -0.3 125,377 + Rv0106 0.84 -0.4 125,470 - +0.0 125,605 + -0.3 125,606 - +0.0 125,620 + -0.2 125,644 + -1.7 125,666 + -0.6
Or see this region's nucleotide sequence