Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2623

Experiment: 7H9 with Nickel (II) chloride 0.6 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2622 and Rv2623 are separated by 135 nucleotidesRv2623 and Rv2624c are separated by 2 nucleotidesRv2624c and Rv2625c are separated by 14 nucleotides Rv2622: Rv2622 - Possible methyltransferase (methylase), at 2,948,636 to 2,949,457 Rv2622 Rv2623: Rv2623 - Universal stress protein family protein TB31.7, at 2,949,593 to 2,950,486 Rv2623 Rv2624c: Rv2624c - Universal stress protein family protein, at 2,950,489 to 2,951,307 Rv2624c Rv2625c: Rv2625c - Probable conserved transmembrane alanine and leucine rich protein, at 2,951,322 to 2,952,503 Rv2625c Position (kb) 2949 2950 2951Strain fitness (log2 ratio) -2 -1 0 1at 2948.635 kb on + strandat 2948.635 kb on + strandat 2948.635 kb on + strandat 2948.635 kb on + strandat 2948.635 kb on + strandat 2948.636 kb on - strandat 2948.873 kb on + strand, within Rv2622at 2948.873 kb on + strand, within Rv2622at 2949.277 kb on - strand, within Rv2622at 2949.485 kb on + strandat 2949.623 kb on - strandat 2949.735 kb on + strand, within Rv2623at 2950.080 kb on + strand, within Rv2623at 2950.081 kb on - strand, within Rv2623at 2950.081 kb on - strand, within Rv2623at 2950.485 kb on - strandat 2950.661 kb on + strand, within Rv2624cat 2951.141 kb on + strand, within Rv2624cat 2951.263 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Nickel (II) chloride 0.6 mM
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2,948,635 + +0.0
2,948,635 + +0.2
2,948,635 + +0.4
2,948,635 + -0.6
2,948,635 + -1.9
2,948,636 - -0.4
2,948,873 + Rv2622 0.29 +0.4
2,948,873 + Rv2622 0.29 -0.8
2,949,277 - Rv2622 0.78 -0.3
2,949,485 + -0.2
2,949,623 - -0.3
2,949,735 + Rv2623 0.16 +0.7
2,950,080 + Rv2623 0.54 +1.0
2,950,081 - Rv2623 0.55 -0.4
2,950,081 - Rv2623 0.55 +0.2
2,950,485 - +0.1
2,950,661 + Rv2624c 0.21 +1.7
2,951,141 + Rv2624c 0.80 -0.2
2,951,263 - +1.2

Or see this region's nucleotide sequence