Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0078B

Experiment: 7H9 with Nickel (II) chloride 0.6 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0078 and Rv0078A are separated by 74 nucleotidesRv0078A and Rv0078B overlap by 4 nucleotidesRv0078B and Rv0079 are separated by 199 nucleotides Rv0078: Rv0078 - Probable transcriptional regulatory protein, at 86,528 to 87,133 Rv0078 Rv0078A: Rv0078A - Hypothetical protein, at 87,208 to 87,801 Rv0078A Rv0078B: Rv0078B - Conserved protein, at 87,798 to 88,004 Rv0078B Rv0079: Rv0079 - Unknown protein, at 88,204 to 89,025 Rv0079 Position (kb) 87 88 89Strain fitness (log2 ratio) -2 -1 0 1 2 3at 86.836 kb on - strand, within Rv0078at 86.925 kb on - strand, within Rv0078at 87.125 kb on - strandat 87.276 kb on + strand, within Rv0078Aat 87.289 kb on - strand, within Rv0078Aat 87.343 kb on + strand, within Rv0078Aat 87.605 kb on + strand, within Rv0078Aat 87.606 kb on - strand, within Rv0078Aat 87.632 kb on - strand, within Rv0078Aat 87.955 kb on + strand, within Rv0078Bat 87.955 kb on + strand, within Rv0078Bat 87.955 kb on + strand, within Rv0078Bat 87.955 kb on + strand, within Rv0078Bat 87.955 kb on + strand, within Rv0078Bat 88.233 kb on - strandat 88.233 kb on - strandat 88.280 kb on + strandat 88.440 kb on - strand, within Rv0079at 88.631 kb on + strand, within Rv0079at 88.681 kb on + strand, within Rv0079at 88.682 kb on - strand, within Rv0079at 88.732 kb on + strand, within Rv0079at 88.779 kb on - strand, within Rv0079at 88.779 kb on - strand, within Rv0079at 88.954 kb on + strandat 88.954 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Nickel (II) chloride 0.6 mM
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86,836 - Rv0078 0.51 -0.9
86,925 - Rv0078 0.66 +1.0
87,125 - +0.1
87,276 + Rv0078A 0.11 -0.3
87,289 - Rv0078A 0.14 -0.6
87,343 + Rv0078A 0.23 -0.5
87,605 + Rv0078A 0.67 -1.3
87,606 - Rv0078A 0.67 -0.3
87,632 - Rv0078A 0.71 -0.6
87,955 + Rv0078B 0.76 -1.0
87,955 + Rv0078B 0.76 -0.4
87,955 + Rv0078B 0.76 -0.8
87,955 + Rv0078B 0.76 -0.5
87,955 + Rv0078B 0.76 -2.5
88,233 - -0.4
88,233 - +3.6
88,280 + +1.8
88,440 - Rv0079 0.29 +0.3
88,631 + Rv0079 0.52 +0.0
88,681 + Rv0079 0.58 -0.8
88,682 - Rv0079 0.58 -0.1
88,732 + Rv0079 0.64 -0.6
88,779 - Rv0079 0.70 -0.2
88,779 - Rv0079 0.70 +1.1
88,954 + +0.3
88,954 + +0.2

Or see this region's nucleotide sequence