Experiment: 7H9 with Geneticin G418 0.35 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv2248 and Rv2249c are separated by 65 nucleotides Rv2249c and Rv2250c overlap by 7 nucleotides Rv2250c and Rv2250A are separated by 47 nucleotides
Rv2248: Rv2248 - Conserved hypothetical protein, at 2,522,360 to 2,523,175
Rv2248
Rv2249c: Rv2249c - Probable glycerol-3-phosphate dehydrogenase GlpD1, at 2,523,241 to 2,524,791
Rv2249c
Rv2250c: Rv2250c - Possible transcriptional regulatory protein, at 2,524,785 to 2,525,354
Rv2250c
Rv2250A: Rv2250A - Possible flavoprotein, at 2,525,402 to 2,525,821
Rv2250A
Rv2251: Rv2251 - Possible flavoprotein, at 2,525,565 to 2,526,992
Rv2251
Position (kb)
2523
2524
2525 Strain fitness (log2 ratio)
-1
0
1
2 at 2522.304 kb on + strand at 2522.578 kb on - strand, within Rv2248 at 2522.736 kb on + strand, within Rv2248 at 2522.827 kb on - strand, within Rv2248 at 2523.328 kb on - strand at 2523.620 kb on + strand, within Rv2249c at 2523.742 kb on + strand, within Rv2249c at 2523.762 kb on - strand, within Rv2249c at 2523.816 kb on + strand, within Rv2249c at 2524.560 kb on - strand, within Rv2249c at 2524.663 kb on + strand at 2524.795 kb on + strand at 2524.796 kb on - strand at 2524.796 kb on - strand at 2524.815 kb on - strand at 2525.020 kb on + strand, within Rv2250c at 2525.103 kb on + strand, within Rv2250c at 2525.594 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Geneticin G418 0.35 mM remove 2,522,304 + +2.0 2,522,578 - Rv2248 0.27 +0.1 2,522,736 + Rv2248 0.46 -0.9 2,522,827 - Rv2248 0.57 -0.2 2,523,328 - -0.5 2,523,620 + Rv2249c 0.24 +2.0 2,523,742 + Rv2249c 0.32 +0.6 2,523,762 - Rv2249c 0.34 -0.1 2,523,816 + Rv2249c 0.37 +1.2 2,524,560 - Rv2249c 0.85 -1.0 2,524,663 + +1.0 2,524,795 + -0.1 2,524,796 - +0.3 2,524,796 - +2.6 2,524,815 - +1.3 2,525,020 + Rv2250c 0.41 +0.3 2,525,103 + Rv2250c 0.56 -0.2 2,525,594 + -0.4
Or see this region's nucleotide sequence