Experiment: 7H9 with Geneticin G418 0.35 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0030 and Rv0031 are separated by 28 nucleotides Rv0031 and Rv0032 are separated by 500 nucleotides Rv0032 and Rv0033 overlap by 4 nucleotides Rv0033 and Rv0034 overlap by 4 nucleotides Rv0034 and Rv0035 overlap by 4 nucleotides
Rv0030: Rv0030 - Conserved hypothetical protein, at 33,224 to 33,553
Rv0030
Rv0031: Rv0031 - Possible remnant of a transposase, at 33,582 to 33,794
Rv0031
Rv0032: Rv0032 - Possible 8-amino-7-oxononanoate synthase BioF2 (AONS) (8-amino-7-ketopelargonate synthase) (7-keto-8-amino-pelargonic acid synthetase) (7-KAP synthetase) (L-alanine--pimelyl CoA ligase), at 34,295 to 36,610
Rv0032
Rv0033: Rv0033 - Probable acyl carrier protein AcpA (ACP), at 36,607 to 36,870
Rv0033
Rv0034: Rv0034 - Conserved hypothetical protein, at 36,867 to 37,262
Rv0034
Rv0035: Rv0035 - Probable fatty-acid-CoA ligase FadD34 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase), at 37,259 to 38,947
Rv0035
Position (kb) 34
35
36
37 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 33.355 kb on + strand, within Rv0030 at 33.360 kb on - strand, within Rv0030 at 33.797 kb on + strand at 33.798 kb on - strand at 33.861 kb on + strand at 33.862 kb on - strand at 34.164 kb on - strand at 34.164 kb on - strand at 34.367 kb on + strand at 34.419 kb on - strand at 34.421 kb on - strand at 34.505 kb on - strand at 34.679 kb on + strand, within Rv0032 at 34.679 kb on + strand, within Rv0032 at 34.679 kb on + strand, within Rv0032 at 34.689 kb on - strand, within Rv0032 at 34.744 kb on - strand, within Rv0032 at 34.937 kb on + strand, within Rv0032 at 34.937 kb on + strand, within Rv0032 at 34.999 kb on + strand, within Rv0032 at 35.076 kb on - strand, within Rv0032 at 35.570 kb on + strand, within Rv0032 at 35.570 kb on + strand, within Rv0032 at 35.703 kb on + strand, within Rv0032 at 35.703 kb on + strand, within Rv0032 at 35.874 kb on - strand, within Rv0032 at 35.910 kb on + strand, within Rv0032 at 35.910 kb on + strand, within Rv0032 at 36.029 kb on + strand, within Rv0032 at 36.029 kb on + strand, within Rv0032 at 36.029 kb on + strand, within Rv0032 at 36.030 kb on - strand, within Rv0032 at 36.030 kb on - strand, within Rv0032 at 36.030 kb on - strand, within Rv0032 at 36.117 kb on + strand, within Rv0032 at 36.192 kb on - strand, within Rv0032 at 36.326 kb on + strand, within Rv0032 at 36.326 kb on + strand, within Rv0032 at 36.326 kb on + strand, within Rv0032 at 36.326 kb on + strand, within Rv0032 at 36.327 kb on - strand, within Rv0032 at 36.327 kb on - strand, within Rv0032 at 36.327 kb on - strand, within Rv0032 at 36.327 kb on - strand, within Rv0032 at 36.356 kb on + strand, within Rv0032 at 36.368 kb on + strand, within Rv0032 at 36.369 kb on - strand, within Rv0032 at 36.428 kb on + strand at 36.455 kb on + strand at 36.455 kb on + strand at 36.455 kb on + strand at 36.455 kb on + strand at 36.455 kb on + strand at 36.456 kb on - strand at 36.456 kb on - strand at 36.520 kb on - strand at 36.606 kb on + strand at 36.607 kb on - strand at 36.678 kb on - strand, within Rv0033 at 36.734 kb on + strand, within Rv0033 at 36.734 kb on + strand, within Rv0033 at 36.735 kb on - strand, within Rv0033 at 36.735 kb on - strand, within Rv0033 at 37.093 kb on + strand, within Rv0034 at 37.094 kb on - strand, within Rv0034 at 37.185 kb on - strand, within Rv0034 at 37.225 kb on - strand at 37.225 kb on - strand at 37.353 kb on + strand at 37.353 kb on + strand at 37.354 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Geneticin G418 0.35 mM remove 33,355 + Rv0030 0.40 +1.1 33,360 - Rv0030 0.41 +0.0 33,797 + -0.1 33,798 - -0.2 33,861 + -0.2 33,862 - +0.5 34,164 - -0.2 34,164 - +1.7 34,367 + -0.5 34,419 - +2.9 34,421 - -0.3 34,505 - +1.8 34,679 + Rv0032 0.17 +0.4 34,679 + Rv0032 0.17 -0.0 34,679 + Rv0032 0.17 +0.5 34,689 - Rv0032 0.17 -0.4 34,744 - Rv0032 0.19 +0.5 34,937 + Rv0032 0.28 +0.4 34,937 + Rv0032 0.28 +3.4 34,999 + Rv0032 0.30 +0.5 35,076 - Rv0032 0.34 +0.2 35,570 + Rv0032 0.55 -0.3 35,570 + Rv0032 0.55 +0.2 35,703 + Rv0032 0.61 +0.1 35,703 + Rv0032 0.61 -0.1 35,874 - Rv0032 0.68 +2.3 35,910 + Rv0032 0.70 +1.4 35,910 + Rv0032 0.70 -0.2 36,029 + Rv0032 0.75 -1.6 36,029 + Rv0032 0.75 +0.0 36,029 + Rv0032 0.75 +2.1 36,030 - Rv0032 0.75 -1.2 36,030 - Rv0032 0.75 -0.1 36,030 - Rv0032 0.75 +0.1 36,117 + Rv0032 0.79 -0.2 36,192 - Rv0032 0.82 +3.6 36,326 + Rv0032 0.88 +0.6 36,326 + Rv0032 0.88 -0.2 36,326 + Rv0032 0.88 +0.3 36,326 + Rv0032 0.88 +0.1 36,327 - Rv0032 0.88 -0.3 36,327 - Rv0032 0.88 -0.9 36,327 - Rv0032 0.88 -1.7 36,327 - Rv0032 0.88 +0.3 36,356 + Rv0032 0.89 +0.4 36,368 + Rv0032 0.90 -0.1 36,369 - Rv0032 0.90 -1.6 36,428 + -0.3 36,455 + +2.1 36,455 + -0.1 36,455 + -1.5 36,455 + +2.1 36,455 + -0.1 36,456 - +2.1 36,456 - -0.2 36,520 - -0.2 36,606 + +0.5 36,607 - +0.3 36,678 - Rv0033 0.27 +0.4 36,734 + Rv0033 0.48 -1.0 36,734 + Rv0033 0.48 +0.1 36,735 - Rv0033 0.48 -0.0 36,735 - Rv0033 0.48 +2.1 37,093 + Rv0034 0.57 +0.6 37,094 - Rv0034 0.57 +0.4 37,185 - Rv0034 0.80 +0.0 37,225 - +2.1 37,225 - -0.2 37,353 + -0.4 37,353 + -2.4 37,354 - -0.0
Or see this region's nucleotide sequence