Experiment: 7H9 with Geneticin G418 0.35 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0046c and Rv0047c are separated by 60 nucleotides Rv0047c and Rv0048c are separated by 100 nucleotides Rv0048c and Rv0049 are separated by 133 nucleotides Rv0049 and Rv0050 are separated by 418 nucleotides
Rv0046c: Rv0046c - myo-inositol-1-phosphate synthase Ino1 (inositol 1-phosphate synthetase) (D-glucose 6-phosphate cycloaldolase) (glucose 6-phosphate cyclase) (glucocycloaldolase), at 50,021 to 51,124
Rv0046c
Rv0047c: Rv0047c - Conserved protein, at 51,185 to 51,727
Rv0047c
Rv0048c: Rv0048c - Possible membrane protein, at 51,828 to 52,697
Rv0048c
Rv0049: Rv0049 - Conserved hypothetical protein, at 52,831 to 53,244
Rv0049
Rv0050: Rv0050 - Probable bifunctional penicillin-binding protein 1A/1B PonA1 (murein polymerase) (PBP1): penicillin-insensitive transglycosylase (peptidoglycan TGASE) + penicillin-sensitive transpeptidase (DD-transpeptidase), at 53,663 to 55,699
Rv0050
Position (kb) 51
52
53 Strain fitness (log2 ratio) -2
-1
0
1
2 at 51.454 kb on - strand, within Rv0047c at 51.454 kb on - strand, within Rv0047c at 51.520 kb on - strand, within Rv0047c at 51.610 kb on - strand, within Rv0047c at 51.612 kb on - strand, within Rv0047c at 51.622 kb on - strand, within Rv0047c at 51.676 kb on - strand at 51.676 kb on - strand at 51.746 kb on - strand at 51.746 kb on - strand at 51.807 kb on - strand at 51.907 kb on + strand at 51.907 kb on + strand at 51.907 kb on + strand at 51.907 kb on + strand at 51.907 kb on + strand at 51.907 kb on + strand at 51.907 kb on + strand at 51.908 kb on - strand at 51.908 kb on - strand at 51.908 kb on - strand at 51.908 kb on - strand at 51.908 kb on - strand at 51.908 kb on - strand at 51.908 kb on - strand at 51.967 kb on + strand, within Rv0048c at 51.967 kb on + strand, within Rv0048c at 52.124 kb on - strand, within Rv0048c at 52.127 kb on + strand, within Rv0048c at 52.127 kb on + strand, within Rv0048c at 52.127 kb on + strand, within Rv0048c at 52.127 kb on + strand, within Rv0048c at 52.127 kb on + strand, within Rv0048c at 52.127 kb on + strand, within Rv0048c at 52.129 kb on + strand, within Rv0048c at 52.180 kb on + strand, within Rv0048c at 52.366 kb on + strand, within Rv0048c at 52.524 kb on + strand, within Rv0048c at 52.525 kb on - strand, within Rv0048c at 52.541 kb on + strand, within Rv0048c at 52.541 kb on + strand, within Rv0048c at 52.542 kb on - strand, within Rv0048c at 52.542 kb on - strand, within Rv0048c at 52.542 kb on - strand, within Rv0048c at 52.659 kb on + strand at 52.706 kb on + strand at 52.706 kb on + strand at 52.706 kb on + strand at 52.707 kb on - strand at 52.707 kb on - strand at 52.709 kb on + strand at 52.746 kb on + strand at 52.775 kb on - strand at 52.797 kb on + strand at 52.798 kb on - strand at 52.829 kb on + strand at 52.829 kb on + strand at 52.837 kb on + strand at 52.837 kb on + strand at 52.837 kb on + strand at 53.114 kb on + strand, within Rv0049 at 53.114 kb on + strand, within Rv0049 at 53.152 kb on + strand, within Rv0049 at 53.152 kb on + strand, within Rv0049 at 53.152 kb on + strand, within Rv0049 at 53.152 kb on + strand, within Rv0049
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Geneticin G418 0.35 mM remove 51,454 - Rv0047c 0.50 +2.5 51,454 - Rv0047c 0.50 +0.6 51,520 - Rv0047c 0.62 -0.7 51,610 - Rv0047c 0.78 -0.4 51,612 - Rv0047c 0.79 +0.1 51,622 - Rv0047c 0.80 -1.4 51,676 - +0.3 51,676 - +0.0 51,746 - +1.8 51,746 - -0.7 51,807 - -0.7 51,907 + -0.2 51,907 + -0.2 51,907 + -0.1 51,907 + -1.0 51,907 + +0.3 51,907 + -0.0 51,907 + +0.1 51,908 - -0.4 51,908 - -1.1 51,908 - +0.4 51,908 - -0.2 51,908 - +0.7 51,908 - -1.0 51,908 - +0.1 51,967 + Rv0048c 0.16 -0.1 51,967 + Rv0048c 0.16 -0.2 52,124 - Rv0048c 0.34 -0.6 52,127 + Rv0048c 0.34 -0.5 52,127 + Rv0048c 0.34 -1.4 52,127 + Rv0048c 0.34 -0.6 52,127 + Rv0048c 0.34 -0.8 52,127 + Rv0048c 0.34 -0.9 52,127 + Rv0048c 0.34 +1.3 52,129 + Rv0048c 0.35 -0.1 52,180 + Rv0048c 0.40 -0.7 52,366 + Rv0048c 0.62 +0.1 52,524 + Rv0048c 0.80 -0.4 52,525 - Rv0048c 0.80 -0.6 52,541 + Rv0048c 0.82 -0.2 52,541 + Rv0048c 0.82 -0.7 52,542 - Rv0048c 0.82 -0.9 52,542 - Rv0048c 0.82 -0.3 52,542 - Rv0048c 0.82 -0.4 52,659 + -0.4 52,706 + -1.5 52,706 + -0.3 52,706 + -0.6 52,707 - -0.7 52,707 - -0.4 52,709 + -1.1 52,746 + +1.3 52,775 - -0.5 52,797 + +0.2 52,798 - +0.6 52,829 + -0.9 52,829 + -0.1 52,837 + -0.5 52,837 + -0.9 52,837 + -1.2 53,114 + Rv0049 0.68 +0.2 53,114 + Rv0049 0.68 +0.5 53,152 + Rv0049 0.78 +1.9 53,152 + Rv0049 0.78 -2.0 53,152 + Rv0049 0.78 -0.6 53,152 + Rv0049 0.78 -0.1
Or see this region's nucleotide sequence