Experiment: 7H9 with Sisomicin 0.07 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv2974c and Rv2975c overlap by 4 nucleotides Rv2975c and Rv2976c are separated by 458 nucleotides Rv2976c and Rv2977c are separated by 32 nucleotides
Rv2974c: Rv2974c - Conserved hypothetical alanine rich protein, at 3,329,949 to 3,331,361
Rv2974c
Rv2975c: Rv2975c - Conserved hypothetical protein, at 3,331,358 to 3,331,612
Rv2975c
Rv2976c: Rv2976c - Probable uracil-DNA glycosylase Ung (UDG), at 3,332,071 to 3,332,754
Rv2976c
Rv2977c: Rv2977c - Probable thiamine-monophosphate kinase ThiL (thiamine-phosphate kinase), at 3,332,787 to 3,333,788
Rv2977c
Position (kb)
3332
3333 Strain fitness (log2 ratio)
-2
-1
0
1 at 3331.166 kb on + strand, within Rv2974c at 3331.442 kb on + strand, within Rv2975c at 3331.442 kb on + strand, within Rv2975c at 3331.613 kb on + strand at 3331.613 kb on + strand at 3331.614 kb on - strand at 3331.614 kb on - strand at 3331.614 kb on - strand at 3331.668 kb on + strand at 3331.669 kb on - strand at 3332.518 kb on - strand, within Rv2976c at 3332.631 kb on - strand, within Rv2976c at 3332.770 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Sisomicin 0.07 mM remove 3,331,166 + Rv2974c 0.86 +0.5 3,331,442 + Rv2975c 0.33 -0.5 3,331,442 + Rv2975c 0.33 +1.0 3,331,613 + +1.7 3,331,613 + +1.7 3,331,614 - +0.3 3,331,614 - -0.1 3,331,614 - +1.7 3,331,668 + -0.5 3,331,669 - -0.4 3,332,518 - Rv2976c 0.65 -0.3 3,332,631 - Rv2976c 0.82 -2.5 3,332,770 + +0.0
Or see this region's nucleotide sequence