Experiment: 7H9 with Sisomicin 0.07 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv2857c and Rv2858c overlap by 4 nucleotides Rv2858c and Rv2859c overlap by 4 nucleotides Rv2859c and Rv2860c overlap by 20 nucleotides
Rv2857c: Rv2857c - Probable short-chain type dehydrogenase/reductase, at 3,168,583 to 3,169,359
Rv2857c
Rv2858c: Rv2858c - Probable aldehyde dehydrogenase AldC, at 3,169,356 to 3,170,723
Rv2858c
Rv2859c: Rv2859c - Possible amidotransferase, at 3,170,720 to 3,171,646
Rv2859c
Rv2860c: Rv2860c - Probable glutamine synthetase GlnA4 (glutamine synthase) (GS-II), at 3,171,627 to 3,173,000
Rv2860c
Position (kb)
3169
3170
3171 Strain fitness (log2 ratio)
-2
-1
0
1 at 3168.600 kb on - strand at 3168.668 kb on - strand, within Rv2857c at 3169.111 kb on + strand, within Rv2857c at 3169.198 kb on + strand, within Rv2857c at 3169.199 kb on - strand, within Rv2857c at 3169.774 kb on + strand, within Rv2858c at 3169.775 kb on - strand, within Rv2858c at 3170.440 kb on + strand, within Rv2858c at 3170.718 kb on - strand at 3170.834 kb on + strand, within Rv2859c at 3170.835 kb on - strand, within Rv2859c at 3170.835 kb on - strand, within Rv2859c at 3170.853 kb on - strand, within Rv2859c at 3171.161 kb on + strand, within Rv2859c at 3171.228 kb on + strand, within Rv2859c at 3171.229 kb on - strand, within Rv2859c at 3171.384 kb on + strand, within Rv2859c at 3171.398 kb on - strand, within Rv2859c at 3171.429 kb on + strand, within Rv2859c at 3171.628 kb on + strand at 3171.628 kb on + strand at 3171.648 kb on + strand at 3171.648 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Sisomicin 0.07 mM remove 3,168,600 - -1.8 3,168,668 - Rv2857c 0.11 +0.3 3,169,111 + Rv2857c 0.68 -0.3 3,169,198 + Rv2857c 0.79 -0.1 3,169,199 - Rv2857c 0.79 -0.2 3,169,774 + Rv2858c 0.31 -0.2 3,169,775 - Rv2858c 0.31 -1.6 3,170,440 + Rv2858c 0.79 -2.2 3,170,718 - -0.0 3,170,834 + Rv2859c 0.12 -0.3 3,170,835 - Rv2859c 0.12 +0.3 3,170,835 - Rv2859c 0.12 +1.0 3,170,853 - Rv2859c 0.14 -0.0 3,171,161 + Rv2859c 0.48 -0.2 3,171,228 + Rv2859c 0.55 -1.2 3,171,229 - Rv2859c 0.55 -0.1 3,171,384 + Rv2859c 0.72 -0.1 3,171,398 - Rv2859c 0.73 +0.3 3,171,429 + Rv2859c 0.76 -0.3 3,171,628 + +0.4 3,171,628 + -0.5 3,171,648 + -0.0 3,171,648 + -0.2
Or see this region's nucleotide sequence