Experiment: 7H9 with Sisomicin 0.07 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0538 and Rv0539 are separated by 56 nucleotides Rv0539 and Rv0540 overlap by 4 nucleotides Rv0540 and Rv0541c are separated by 20 nucleotides
Rv0538: Rv0538 - Possible conserved membrane protein, at 630,040 to 631,686
Rv0538
Rv0539: Rv0539 - Probable dolichyl-phosphate sugar synthase (dolichol-phosphate sugar synthetase) (dolichol-phosphate sugar transferase) (sugar phosphoryldolichol synthase), at 631,743 to 632,375
Rv0539
Rv0540: Rv0540 - Conserved hypothetical protein, at 632,372 to 633,034
Rv0540
Rv0541c: Rv0541c - Probable conserved integral membrane protein, at 633,055 to 634,404
Rv0541c
Position (kb) 632
633
634 Strain fitness (log2 ratio) -2
-1
0
1 at 631.670 kb on + strand at 631.670 kb on + strand at 631.670 kb on + strand at 631.670 kb on + strand at 631.670 kb on + strand at 632.283 kb on + strand, within Rv0539 at 632.284 kb on - strand, within Rv0539 at 632.552 kb on - strand, within Rv0540 at 632.602 kb on + strand, within Rv0540 at 632.603 kb on - strand, within Rv0540 at 632.827 kb on + strand, within Rv0540 at 632.827 kb on + strand, within Rv0540 at 633.057 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Sisomicin 0.07 mM remove 631,670 + -0.0 631,670 + -0.3 631,670 + +0.8 631,670 + -0.3 631,670 + -1.3 632,283 + Rv0539 0.85 -0.3 632,284 - Rv0539 0.85 -0.0 632,552 - Rv0540 0.27 -0.8 632,602 + Rv0540 0.35 -2.0 632,603 - Rv0540 0.35 -2.4 632,827 + Rv0540 0.69 -0.1 632,827 + Rv0540 0.69 -0.5 633,057 - -0.4
Or see this region's nucleotide sequence