Experiment: 7H9 with Sisomicin 0.07 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0186A and Rv0187 are separated by 153 nucleotides Rv0187 and Rv0188 are separated by 118 nucleotides Rv0188 and Rv0189c are separated by 78 nucleotides
Rv0186A: Rv0186A - Metallothionein, MymT, at 218,390 to 218,551
Rv0186A
Rv0187: Rv0187 - Probable O-methyltransferase, at 218,705 to 219,367
Rv0187
Rv0188: Rv0188 - Probable conserved transmembrane protein, at 219,486 to 219,917
Rv0188
Rv0189c: Rv0189c - Probable dihydroxy-acid dehydratase IlvD (dad), at 219,996 to 221,723
Rv0189c
Position (kb) 219
220 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 218.596 kb on + strand at 218.610 kb on + strand at 218.614 kb on + strand at 218.615 kb on - strand at 218.820 kb on + strand, within Rv0187 at 218.981 kb on + strand, within Rv0187 at 218.981 kb on + strand, within Rv0187 at 218.982 kb on - strand, within Rv0187 at 219.148 kb on + strand, within Rv0187 at 219.205 kb on + strand, within Rv0187 at 219.206 kb on - strand, within Rv0187 at 219.298 kb on - strand, within Rv0187 at 219.492 kb on - strand at 219.613 kb on - strand, within Rv0188 at 219.860 kb on - strand, within Rv0188 at 219.915 kb on + strand at 219.916 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Sisomicin 0.07 mM remove 218,596 + -1.4 218,610 + +1.7 218,614 + +0.6 218,615 - -0.9 218,820 + Rv0187 0.17 +1.3 218,981 + Rv0187 0.42 -0.6 218,981 + Rv0187 0.42 +1.7 218,982 - Rv0187 0.42 +2.1 219,148 + Rv0187 0.67 +0.7 219,205 + Rv0187 0.75 +1.4 219,206 - Rv0187 0.76 -0.3 219,298 - Rv0187 0.89 +0.2 219,492 - -0.3 219,613 - Rv0188 0.29 -2.9 219,860 - Rv0188 0.87 -0.7 219,915 + +0.8 219,916 - -0.5
Or see this region's nucleotide sequence