Experiment: 7H9 with Sisomicin 0.07 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0174 and Rv0175 are separated by 35 nucleotides Rv0175 and Rv0176 overlap by 4 nucleotides Rv0176 and Rv0177 overlap by 4 nucleotides Rv0177 and Rv0178 overlap by 34 nucleotides
Rv0174: Rv0174 - Mce-family protein Mce1F, at 205,231 to 206,778
Rv0174
Rv0175: Rv0175 - Probable conserved Mce associated membrane protein, at 206,814 to 207,455
Rv0175
Rv0176: Rv0176 - Probable conserved Mce associated transmembrane protein, at 207,452 to 208,420
Rv0176
Rv0177: Rv0177 - Probable conserved Mce associated protein, at 208,417 to 208,971
Rv0177
Rv0178: Rv0178 - Probable conserved Mce associated membrane protein, at 208,938 to 209,672
Rv0178
Position (kb) 207
208
209 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 206.623 kb on - strand, within Rv0174 at 206.623 kb on - strand, within Rv0174 at 206.636 kb on - strand at 206.711 kb on - strand at 206.711 kb on - strand at 206.795 kb on + strand at 206.808 kb on - strand at 207.420 kb on + strand at 207.422 kb on + strand at 207.423 kb on - strand at 207.423 kb on - strand at 208.010 kb on + strand, within Rv0176 at 208.010 kb on + strand, within Rv0176 at 208.092 kb on - strand, within Rv0176 at 208.152 kb on - strand, within Rv0176 at 208.208 kb on - strand, within Rv0176 at 208.295 kb on - strand, within Rv0176 at 208.295 kb on - strand, within Rv0176 at 208.314 kb on - strand, within Rv0176 at 208.315 kb on + strand, within Rv0176 at 208.316 kb on - strand, within Rv0176 at 208.726 kb on + strand, within Rv0177 at 208.827 kb on + strand, within Rv0177 at 208.828 kb on - strand, within Rv0177 at 209.247 kb on + strand, within Rv0178
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Sisomicin 0.07 mM remove 206,623 - Rv0174 0.90 +1.6 206,623 - Rv0174 0.90 +1.0 206,636 - +1.7 206,711 - +4.5 206,711 - +1.4 206,795 + +1.3 206,808 - +0.4 207,420 + +0.7 207,422 + +1.9 207,423 - +1.7 207,423 - +2.6 208,010 + Rv0176 0.58 +0.7 208,010 + Rv0176 0.58 +1.0 208,092 - Rv0176 0.66 +1.9 208,152 - Rv0176 0.72 +2.0 208,208 - Rv0176 0.78 +1.9 208,295 - Rv0176 0.87 +2.1 208,295 - Rv0176 0.87 +1.0 208,314 - Rv0176 0.89 +1.2 208,315 + Rv0176 0.89 +0.6 208,316 - Rv0176 0.89 +1.2 208,726 + Rv0177 0.56 +0.6 208,827 + Rv0177 0.74 -0.0 208,828 - Rv0177 0.74 -0.4 209,247 + Rv0178 0.42 +1.8
Or see this region's nucleotide sequence