Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2532c

Experiment: 7H9 with Sisomicin 0.07 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2531c and Rv2532c are separated by 71 nucleotidesRv2532c and Rv2533c overlap by 1 nucleotidesRv2533c and Rv2534c are separated by 2 nucleotides Rv2531c: Rv2531c - Probable amino acid decarboxylase, at 2,854,938 to 2,857,781 Rv2531c Rv2532c: Rv2532c - Hypothetical protein, at 2,857,853 to 2,858,254 Rv2532c Rv2533c: Rv2533c - N utilization substance protein NusB (NusB protein), at 2,858,254 to 2,858,724 Rv2533c Rv2534c: Rv2534c - Probable elongation factor P Efp, at 2,858,727 to 2,859,290 Rv2534c Position (kb) 2857 2858 2859Strain fitness (log2 ratio) -1 0 1 2 3at 2857.476 kb on + strand, within Rv2531cat 2857.477 kb on - strand, within Rv2531cat 2857.564 kb on + strandat 2857.582 kb on + strandat 2857.711 kb on + strandat 2857.712 kb on - strandat 2857.712 kb on - strandat 2857.712 kb on - strandat 2857.815 kb on + strandat 2857.816 kb on - strandat 2857.816 kb on - strandat 2857.834 kb on + strandat 2857.862 kb on + strandat 2857.862 kb on + strandat 2857.862 kb on + strandat 2857.863 kb on - strandat 2857.863 kb on - strandat 2858.110 kb on - strand, within Rv2532cat 2858.136 kb on + strand, within Rv2532cat 2858.137 kb on - strand, within Rv2532c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Sisomicin 0.07 mM
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2,857,476 + Rv2531c 0.89 -0.2
2,857,477 - Rv2531c 0.89 +0.3
2,857,564 + +1.0
2,857,582 + -0.5
2,857,711 + -0.6
2,857,712 - -0.3
2,857,712 - +1.2
2,857,712 - -1.4
2,857,815 + +0.2
2,857,816 - +0.4
2,857,816 - -0.7
2,857,834 + +1.2
2,857,862 + +2.9
2,857,862 + -0.9
2,857,862 + -0.9
2,857,863 - +0.6
2,857,863 - +0.1
2,858,110 - Rv2532c 0.64 -0.7
2,858,136 + Rv2532c 0.70 -0.3
2,858,137 - Rv2532c 0.71 +1.5

Or see this region's nucleotide sequence