Experiment: 7H9 with Sisomicin 0.07 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0658c and Rv0659c are separated by 275 nucleotides Rv0659c and Rv0660c overlap by 14 nucleotides Rv0660c and Rv0661c are separated by 109 nucleotides Rv0661c and Rv0662c overlap by 4 nucleotides
Rv0658c: Rv0658c - Probable conserved integral membrane protein, at 753,693 to 754,409
Rv0658c
Rv0659c: Rv0659c - Toxin MazF2, at 754,685 to 754,993
Rv0659c
Rv0660c: Rv0660c - Possible antitoxin MazE2, at 754,980 to 755,225
Rv0660c
Rv0661c: Rv0661c - Possible toxin VapC7, at 755,335 to 755,772
Rv0661c
Rv0662c: Rv0662c - Possible antitoxin VapB7, at 755,769 to 756,023
Rv0662c
Position (kb)
754
755 Strain fitness (log2 ratio)
-1
0
1
2
3 at 753.706 kb on - strand at 753.732 kb on + strand at 753.747 kb on + strand at 753.783 kb on + strand, within Rv0658c at 753.797 kb on - strand, within Rv0658c at 754.047 kb on + strand, within Rv0658c at 754.332 kb on + strand, within Rv0658c at 754.383 kb on + strand at 754.487 kb on + strand at 754.487 kb on + strand at 754.783 kb on + strand, within Rv0659c at 754.783 kb on + strand, within Rv0659c at 754.783 kb on + strand, within Rv0659c at 754.784 kb on - strand, within Rv0659c at 754.784 kb on - strand, within Rv0659c at 754.941 kb on - strand, within Rv0659c at 755.080 kb on + strand, within Rv0660c at 755.080 kb on + strand, within Rv0660c at 755.081 kb on - strand, within Rv0660c at 755.249 kb on + strand at 755.249 kb on + strand at 755.250 kb on - strand at 755.250 kb on - strand at 755.250 kb on - strand at 755.280 kb on - strand at 755.280 kb on - strand at 755.280 kb on - strand at 755.311 kb on + strand at 755.311 kb on + strand at 755.572 kb on - strand, within Rv0661c at 755.623 kb on + strand, within Rv0661c at 755.767 kb on + strand at 755.768 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Sisomicin 0.07 mM remove 753,706 - +0.8 753,732 + +0.6 753,747 + -0.6 753,783 + Rv0658c 0.13 -0.0 753,797 - Rv0658c 0.15 -0.4 754,047 + Rv0658c 0.49 +0.3 754,332 + Rv0658c 0.89 +0.3 754,383 + +2.6 754,487 + -0.3 754,487 + -0.4 754,783 + Rv0659c 0.32 -1.3 754,783 + Rv0659c 0.32 -1.4 754,783 + Rv0659c 0.32 -1.0 754,784 - Rv0659c 0.32 +0.9 754,784 - Rv0659c 0.32 -0.5 754,941 - Rv0659c 0.83 -1.2 755,080 + Rv0660c 0.41 +3.0 755,080 + Rv0660c 0.41 +0.4 755,081 - Rv0660c 0.41 -0.1 755,249 + +0.0 755,249 + -0.5 755,250 - -1.5 755,250 - -1.1 755,250 - -0.6 755,280 - -0.0 755,280 - +0.6 755,280 - -0.8 755,311 + -0.4 755,311 + -0.8 755,572 - Rv0661c 0.54 -1.1 755,623 + Rv0661c 0.66 -0.2 755,767 + -0.8 755,768 - +0.8
Or see this region's nucleotide sequence