Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3213c

Experiment: 7H9 with nitrate 4 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3212 and Rv3213c are separated by 74 nucleotidesRv3213c and Rv3214 are separated by 153 nucleotidesRv3214 and Rv3215 overlap by 4 nucleotides Rv3212: Rv3212 - Conserved alanine valine rich protein, at 3,589,394 to 3,590,617 Rv3212 Rv3213c: Rv3213c - Possible SOJ/para-related protein, at 3,590,692 to 3,591,492 Rv3213c Rv3214: Rv3214 - Possible phosphoglycerate mutase Gpm2 (phosphoglyceromutase) (PGAM) (BPG-dependent PGAM), at 3,591,646 to 3,592,257 Rv3214 Rv3215: Rv3215 - Probable isochorismate synthase EntC (isochorismate hydroxymutase) (enterochelin biosynthesis), at 3,592,254 to 3,593,372 Rv3215 Position (kb) 3590 3591 3592Strain fitness (log2 ratio) -1 0 1 2at 3590.623 kb on + strandat 3590.724 kb on + strandat 3590.724 kb on + strandat 3590.933 kb on + strand, within Rv3213cat 3590.933 kb on + strand, within Rv3213cat 3590.934 kb on - strand, within Rv3213cat 3590.934 kb on - strand, within Rv3213cat 3590.934 kb on - strand, within Rv3213cat 3591.320 kb on - strand, within Rv3213cat 3591.432 kb on + strandat 3591.493 kb on - strandat 3591.493 kb on - strandat 3591.617 kb on + strandat 3591.618 kb on - strandat 3591.881 kb on + strand, within Rv3214

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with nitrate 4 mM
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3,590,623 + -0.9
3,590,724 + -0.3
3,590,724 + -0.1
3,590,933 + Rv3213c 0.30 -0.4
3,590,933 + Rv3213c 0.30 -1.4
3,590,934 - Rv3213c 0.30 -0.0
3,590,934 - Rv3213c 0.30 +2.4
3,590,934 - Rv3213c 0.30 -1.4
3,591,320 - Rv3213c 0.78 -1.1
3,591,432 + -0.1
3,591,493 - +0.5
3,591,493 - +0.4
3,591,617 + -0.0
3,591,618 - +0.1
3,591,881 + Rv3214 0.38 -0.4

Or see this region's nucleotide sequence