Experiment: 7H9 with nitrite 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0299 and Rv0300 are separated by 47 nucleotides Rv0300 and Rv0301 overlap by 4 nucleotides Rv0301 and Rv0302 are separated by 135 nucleotides Rv0302 and Rv0303 overlap by 4 nucleotides Rv0303 and Rv0304c are separated by 7 nucleotides
Rv0299: Rv0299 - Hypothetical protein, at 363,476 to 363,778
Rv0299
Rv0300: Rv0300 - Possible antitoxin VapB2, at 363,826 to 364,047
Rv0300
Rv0301: Rv0301 - Possible toxin VapC2, at 364,044 to 364,469
Rv0301
Rv0302: Rv0302 - Probable transcriptional regulatory protein (probably TetR/AcrR-family), at 364,605 to 365,237
Rv0302
Rv0303: Rv0303 - Probable dehydrogenase/reductase, at 365,234 to 366,142
Rv0303
Rv0304c: Rv0304c - PPE family protein PPE5, at 366,150 to 372,764
Rv0304c
Position (kb) 364
365
366 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 363.814 kb on + strand at 363.815 kb on - strand at 363.836 kb on + strand at 363.837 kb on - strand at 363.837 kb on - strand at 363.866 kb on + strand, within Rv0300 at 363.866 kb on + strand, within Rv0300 at 363.867 kb on - strand, within Rv0300 at 364.027 kb on - strand at 364.144 kb on + strand, within Rv0301 at 364.145 kb on - strand, within Rv0301 at 364.145 kb on - strand, within Rv0301 at 364.145 kb on - strand, within Rv0301 at 364.174 kb on + strand, within Rv0301 at 364.175 kb on - strand, within Rv0301 at 364.175 kb on - strand, within Rv0301 at 364.251 kb on + strand, within Rv0301 at 364.251 kb on + strand, within Rv0301 at 364.252 kb on - strand, within Rv0301 at 364.252 kb on - strand, within Rv0301 at 364.252 kb on - strand, within Rv0301 at 364.256 kb on - strand, within Rv0301 at 364.256 kb on - strand, within Rv0301 at 364.256 kb on - strand, within Rv0301 at 364.381 kb on + strand, within Rv0301 at 364.382 kb on - strand, within Rv0301 at 364.382 kb on - strand, within Rv0301 at 364.568 kb on - strand at 364.741 kb on + strand, within Rv0302 at 364.755 kb on - strand, within Rv0302 at 364.755 kb on - strand, within Rv0302 at 364.758 kb on + strand, within Rv0302 at 364.942 kb on - strand, within Rv0302 at 365.131 kb on - strand, within Rv0302 at 365.178 kb on + strand at 365.179 kb on - strand at 365.179 kb on - strand at 365.304 kb on + strand at 365.565 kb on + strand, within Rv0303 at 365.693 kb on + strand, within Rv0303 at 365.695 kb on + strand, within Rv0303 at 365.696 kb on - strand, within Rv0303 at 365.696 kb on - strand, within Rv0303 at 365.696 kb on - strand, within Rv0303 at 365.762 kb on + strand, within Rv0303 at 365.762 kb on + strand, within Rv0303 at 365.798 kb on + strand, within Rv0303 at 365.799 kb on - strand, within Rv0303 at 365.865 kb on + strand, within Rv0303 at 365.918 kb on + strand, within Rv0303 at 365.918 kb on + strand, within Rv0303 at 366.077 kb on + strand at 366.140 kb on + strand at 366.140 kb on + strand at 366.140 kb on + strand at 366.141 kb on - strand at 366.141 kb on - strand at 366.141 kb on - strand at 366.141 kb on - strand at 366.159 kb on - strand at 366.189 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with nitrite 5 mM remove 363,814 + -0.5 363,815 - -0.6 363,836 + +0.2 363,837 - -0.7 363,837 - +0.2 363,866 + Rv0300 0.18 -0.3 363,866 + Rv0300 0.18 -2.2 363,867 - Rv0300 0.18 -0.7 364,027 - -0.6 364,144 + Rv0301 0.23 -0.3 364,145 - Rv0301 0.24 -0.3 364,145 - Rv0301 0.24 +0.2 364,145 - Rv0301 0.24 -0.6 364,174 + Rv0301 0.31 -0.2 364,175 - Rv0301 0.31 -0.2 364,175 - Rv0301 0.31 -0.0 364,251 + Rv0301 0.49 -0.3 364,251 + Rv0301 0.49 +0.4 364,252 - Rv0301 0.49 -0.3 364,252 - Rv0301 0.49 -0.8 364,252 - Rv0301 0.49 +0.7 364,256 - Rv0301 0.50 +0.2 364,256 - Rv0301 0.50 -0.5 364,256 - Rv0301 0.50 -0.2 364,381 + Rv0301 0.79 +0.4 364,382 - Rv0301 0.79 -0.6 364,382 - Rv0301 0.79 -0.6 364,568 - -0.7 364,741 + Rv0302 0.21 -0.9 364,755 - Rv0302 0.24 -0.5 364,755 - Rv0302 0.24 -0.4 364,758 + Rv0302 0.24 -0.7 364,942 - Rv0302 0.53 +0.2 365,131 - Rv0302 0.83 -0.8 365,178 + -0.3 365,179 - +0.7 365,179 - -0.8 365,304 + -0.6 365,565 + Rv0303 0.36 -0.2 365,693 + Rv0303 0.50 -0.1 365,695 + Rv0303 0.51 +0.2 365,696 - Rv0303 0.51 -1.1 365,696 - Rv0303 0.51 +0.4 365,696 - Rv0303 0.51 +0.1 365,762 + Rv0303 0.58 -0.9 365,762 + Rv0303 0.58 +0.4 365,798 + Rv0303 0.62 -3.1 365,799 - Rv0303 0.62 -1.2 365,865 + Rv0303 0.69 +1.7 365,918 + Rv0303 0.75 -1.4 365,918 + Rv0303 0.75 -0.2 366,077 + -0.4 366,140 + -0.3 366,140 + -0.8 366,140 + -0.0 366,141 - +0.5 366,141 - -0.5 366,141 - +0.3 366,141 - -0.8 366,159 - -1.1 366,189 - +0.1
Or see this region's nucleotide sequence