Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2466c

Experiment: 7H9 with nitrite 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2464c and Rv2465c are separated by 5 nucleotidesRv2465c and Rv2466c are separated by 101 nucleotidesRv2466c and Rv2467 are separated by 101 nucleotides Rv2464c: Rv2464c - Possible DNA glycosylase, at 2,766,859 to 2,767,665 Rv2464c Rv2465c: Rv2465c - Ribose-5-phosphate isomerase, at 2,767,671 to 2,768,159 Rv2465c Rv2466c: Rv2466c - Conserved protein, at 2,768,261 to 2,768,884 Rv2466c Rv2467: Rv2467 - Probable aminopeptidase N PepN (Lysyl aminopeptidase) (LYS-AP) (alanine aminopeptidase), at 2,768,986 to 2,771,571 Rv2467 Position (kb) 2768 2769Strain fitness (log2 ratio) -2 -1 0 1 2at 2767.454 kb on + strand, within Rv2464cat 2767.455 kb on - strand, within Rv2464cat 2767.455 kb on - strand, within Rv2464cat 2767.493 kb on + strand, within Rv2464cat 2767.493 kb on + strand, within Rv2464cat 2767.494 kb on - strand, within Rv2464cat 2767.650 kb on + strandat 2767.650 kb on + strandat 2767.651 kb on - strandat 2768.262 kb on + strandat 2768.316 kb on + strandat 2768.329 kb on + strand, within Rv2466cat 2768.330 kb on - strand, within Rv2466cat 2768.330 kb on - strand, within Rv2466cat 2768.429 kb on - strand, within Rv2466cat 2768.613 kb on + strand, within Rv2466cat 2768.738 kb on + strand, within Rv2466cat 2768.920 kb on + strandat 2769.052 kb on + strandat 2769.053 kb on - strandat 2769.301 kb on + strand, within Rv2467at 2769.358 kb on + strand, within Rv2467at 2769.529 kb on - strand, within Rv2467at 2769.529 kb on - strand, within Rv2467at 2769.595 kb on + strand, within Rv2467at 2769.596 kb on - strand, within Rv2467at 2769.656 kb on - strand, within Rv2467at 2769.706 kb on + strand, within Rv2467at 2769.853 kb on + strand, within Rv2467

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with nitrite 2.5 mM
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2,767,454 + Rv2464c 0.74 -0.1
2,767,455 - Rv2464c 0.74 +0.9
2,767,455 - Rv2464c 0.74 +0.3
2,767,493 + Rv2464c 0.79 -1.0
2,767,493 + Rv2464c 0.79 -1.8
2,767,494 - Rv2464c 0.79 -1.2
2,767,650 + -1.0
2,767,650 + -0.5
2,767,651 - +0.4
2,768,262 + -1.0
2,768,316 + +1.7
2,768,329 + Rv2466c 0.11 +0.4
2,768,330 - Rv2466c 0.11 -0.8
2,768,330 - Rv2466c 0.11 -1.8
2,768,429 - Rv2466c 0.27 -1.0
2,768,613 + Rv2466c 0.56 -0.3
2,768,738 + Rv2466c 0.76 -0.6
2,768,920 + -0.5
2,769,052 + -0.6
2,769,053 - -0.5
2,769,301 + Rv2467 0.12 -0.6
2,769,358 + Rv2467 0.14 -0.5
2,769,529 - Rv2467 0.21 +0.4
2,769,529 - Rv2467 0.21 +2.1
2,769,595 + Rv2467 0.24 +1.2
2,769,596 - Rv2467 0.24 +0.4
2,769,656 - Rv2467 0.26 -0.6
2,769,706 + Rv2467 0.28 -0.3
2,769,853 + Rv2467 0.34 +0.1

Or see this region's nucleotide sequence