Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2274c

Experiment: 7H9 with nitrite 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2271 and Rv2272 are separated by 105 nucleotidesRv2272 and Rv2273 overlap by 4 nucleotidesRv2273 and Rv2274c are separated by 56 nucleotidesRv2274c and Rv2274A are separated by 33 nucleotidesRv2274A and Rv2275 overlap by 205 nucleotidesRv2275 and Rv2276 overlap by 4 nucleotides Rv2271: Rv2271 - Conserved hypothetical protein, at 2,545,332 to 2,545,631 Rv2271 Rv2272: Rv2272 - Probable conserved transmembrane protein, at 2,545,737 to 2,546,105 Rv2272 Rv2273: Rv2273 - Probable conserved transmembrane protein, at 2,546,102 to 2,546,431 Rv2273 Rv2274c: Rv2274c - Possible toxin MazF8, at 2,546,488 to 2,546,805 Rv2274c Rv2274A: Rv2274A - Possible antitoxin MazE8, at 2,546,839 to 2,547,087 Rv2274A Rv2275: Rv2275 - Conserved hypothetical protein, at 2,546,883 to 2,547,752 Rv2275 Rv2276: Rv2276 - Cytochrome P450 121 Cyp121, at 2,547,749 to 2,548,939 Rv2276 Position (kb) 2546 2547Strain fitness (log2 ratio) -1 0 1at 2545.578 kb on + strand, within Rv2271at 2545.597 kb on + strand, within Rv2271at 2545.598 kb on - strand, within Rv2271at 2545.654 kb on + strandat 2545.655 kb on - strandat 2545.655 kb on - strandat 2545.682 kb on + strandat 2546.032 kb on - strand, within Rv2272at 2546.291 kb on + strand, within Rv2273at 2546.291 kb on + strand, within Rv2273at 2546.291 kb on + strand, within Rv2273at 2546.305 kb on - strand, within Rv2273at 2546.490 kb on - strandat 2546.707 kb on + strand, within Rv2274cat 2546.708 kb on - strand, within Rv2274cat 2546.779 kb on - strandat 2546.800 kb on - strandat 2547.160 kb on - strand, within Rv2275at 2547.567 kb on + strand, within Rv2275at 2547.568 kb on - strand, within Rv2275at 2547.672 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with nitrite 2.5 mM
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2,545,578 + Rv2271 0.82 -0.9
2,545,597 + Rv2271 0.88 -0.6
2,545,598 - Rv2271 0.89 -1.2
2,545,654 + -0.7
2,545,655 - -0.6
2,545,655 - +0.0
2,545,682 + +1.5
2,546,032 - Rv2272 0.80 -0.3
2,546,291 + Rv2273 0.57 -0.5
2,546,291 + Rv2273 0.57 -0.8
2,546,291 + Rv2273 0.57 -0.5
2,546,305 - Rv2273 0.62 -0.1
2,546,490 - +0.9
2,546,707 + Rv2274c 0.69 -1.3
2,546,708 - Rv2274c 0.69 +0.9
2,546,779 - +0.1
2,546,800 - -0.1
2,547,160 - Rv2275 0.32 -0.1
2,547,567 + Rv2275 0.79 -0.3
2,547,568 - Rv2275 0.79 -1.1
2,547,672 + -0.5

Or see this region's nucleotide sequence