Experiment: 7H9 with nitrite 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv1938 and Rv1939 overlap by 4 nucleotides Rv1939 and Rv1940 overlap by 4 nucleotides Rv1940 and Rv1941 overlap by 4 nucleotides Rv1941 and Rv1942c are separated by 209 nucleotides
Rv1938: Rv1938 - Probable epoxide hydrolase EphB (epoxide hydratase), at 2,191,027 to 2,192,097
Rv1938
Rv1939: Rv1939 - Probable oxidoreductase, at 2,192,094 to 2,192,609
Rv1939
Rv1940: Rv1940 - Probable riboflavin biosynthesis protein RibA1 (GTP cyclohydrolase II), at 2,192,606 to 2,193,667
Rv1940
Rv1941: Rv1941 - Probable short-chain type dehydrogenase/reductase, at 2,193,664 to 2,194,434
Rv1941
Rv1942c: Rv1942c - Possible toxin MazF5, at 2,194,644 to 2,194,973
Rv1942c
Position (kb)
2192
2193
2194 Strain fitness (log2 ratio)
-2
-1
0
1 at 2191.708 kb on - strand, within Rv1938 at 2191.927 kb on + strand, within Rv1938 at 2192.372 kb on + strand, within Rv1939 at 2192.372 kb on + strand, within Rv1939 at 2192.373 kb on - strand, within Rv1939 at 2192.700 kb on - strand at 2193.465 kb on - strand, within Rv1940 at 2193.465 kb on - strand, within Rv1940 at 2193.465 kb on - strand, within Rv1940 at 2193.575 kb on + strand at 2193.575 kb on + strand at 2193.575 kb on + strand at 2193.575 kb on + strand at 2193.576 kb on - strand at 2193.576 kb on - strand at 2193.576 kb on - strand at 2194.124 kb on - strand, within Rv1941 at 2194.493 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with nitrite 2.5 mM remove 2,191,708 - Rv1938 0.64 +0.4 2,191,927 + Rv1938 0.84 -0.3 2,192,372 + Rv1939 0.54 -0.1 2,192,372 + Rv1939 0.54 -0.6 2,192,373 - Rv1939 0.54 -0.3 2,192,700 - -1.4 2,193,465 - Rv1940 0.81 -0.7 2,193,465 - Rv1940 0.81 -2.1 2,193,465 - Rv1940 0.81 -0.1 2,193,575 + +0.5 2,193,575 + -1.8 2,193,575 + -0.3 2,193,575 + -0.6 2,193,576 - -0.5 2,193,576 - -0.7 2,193,576 - +0.6 2,194,124 - Rv1941 0.60 +0.1 2,194,493 + +0.2
Or see this region's nucleotide sequence