Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0782

Experiment: 7H9 with nitrite 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0780 and Rv0781 overlap by 4 nucleotidesRv0781 and Rv0782 overlap by 212 nucleotidesRv0782 and Rv0783c are separated by 427 nucleotides Rv0780: Rv0780 - Phosphoribosylaminoimidazole-succinocarboxamide synthase PurC (SAICAR synthetase), at 873,343 to 874,236 Rv0780 Rv0781: Rv0781 - Probable protease II PtrBa [first part] (oligopeptidase B), at 874,233 to 874,943 Rv0781 Rv0782: Rv0782 - Probable protease II PtrBb [second part] (oligopeptidase B), at 874,732 to 876,390 Rv0782 Rv0783c: Rv0783c - Possible multidrug resistance integral membrane efflux protein EmrB, at 876,818 to 878,440 Rv0783c Position (kb) 874 875 876 877Strain fitness (log2 ratio) -1 0 1 2 3at 874.540 kb on + strand, within Rv0781at 874.713 kb on + strand, within Rv0781at 875.285 kb on - strand, within Rv0782at 875.496 kb on + strand, within Rv0782at 875.546 kb on - strand, within Rv0782at 875.860 kb on - strand, within Rv0782at 876.085 kb on + strand, within Rv0782at 876.320 kb on + strandat 876.325 kb on - strandat 876.419 kb on - strandat 876.462 kb on + strandat 877.269 kb on - strand, within Rv0783c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with nitrite 2.5 mM
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874,540 + Rv0781 0.43 -0.4
874,713 + Rv0781 0.68 -0.9
875,285 - Rv0782 0.33 -0.7
875,496 + Rv0782 0.46 -1.0
875,546 - Rv0782 0.49 -0.2
875,860 - Rv0782 0.68 -0.4
876,085 + Rv0782 0.82 +2.9
876,320 + +1.6
876,325 - -0.3
876,419 - +0.3
876,462 + -0.1
877,269 - Rv0783c 0.28 +0.9

Or see this region's nucleotide sequence