Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0589

Experiment: 7H9 with nitrite 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0587 and Rv0588 are separated by 1 nucleotidesRv0588 and Rv0589 are separated by 5 nucleotidesRv0589 and Rv0590 overlap by 4 nucleotidesRv0590 and Rv0590A overlap by 52 nucleotides Rv0587: Rv0587 - Conserved hypothetical integral membrane protein YrbE2A, at 685,129 to 685,926 Rv0587 Rv0588: Rv0588 - Conserved hypothetical integral membrane protein YrbE2B, at 685,928 to 686,815 Rv0588 Rv0589: Rv0589 - Mce-family protein Mce2A, at 686,821 to 688,035 Rv0589 Rv0590: Rv0590 - Mce-family protein Mce2B, at 688,032 to 688,859 Rv0590 Rv0590A: Rv0590A - Mce-family related protein, at 688,808 to 689,062 Rv0590A Position (kb) 686 687 688 689Strain fitness (log2 ratio) -2 -1 0 1at 686.544 kb on - strand, within Rv0588at 686.573 kb on + strand, within Rv0588at 686.663 kb on + strand, within Rv0588at 686.667 kb on - strand, within Rv0588at 686.673 kb on - strand, within Rv0588at 686.781 kb on - strandat 686.863 kb on + strandat 686.864 kb on - strandat 686.988 kb on + strand, within Rv0589at 687.634 kb on + strand, within Rv0589at 687.688 kb on + strand, within Rv0589at 687.991 kb on + strandat 687.991 kb on + strandat 688.235 kb on - strand, within Rv0590at 688.235 kb on - strand, within Rv0590at 688.937 kb on + strand, within Rv0590Aat 688.938 kb on - strand, within Rv0590Aat 689.011 kb on - strand, within Rv0590A

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with nitrite 2.5 mM
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686,544 - Rv0588 0.69 -0.3
686,573 + Rv0588 0.73 +0.2
686,663 + Rv0588 0.83 -0.9
686,667 - Rv0588 0.83 -0.7
686,673 - Rv0588 0.84 -0.6
686,781 - -1.7
686,863 + +1.1
686,864 - -0.4
686,988 + Rv0589 0.14 -0.2
687,634 + Rv0589 0.67 +0.0
687,688 + Rv0589 0.71 -0.4
687,991 + +0.2
687,991 + -2.0
688,235 - Rv0590 0.25 -2.6
688,235 - Rv0590 0.25 -0.5
688,937 + Rv0590A 0.51 +0.1
688,938 - Rv0590A 0.51 -0.5
689,011 - Rv0590A 0.80 -0.4

Or see this region's nucleotide sequence